Bregier, C.a , Krzemień-Ojak, L.a , W?oga, D.a , Jerka-Dziadosz, M.a , Joachimiak, E.a , Batko, K.a , Filipiuk, I.a , ?mietanka, U.a , Gaertig, J.b , Fabczak, S.a , Fabczak, H.a PHLP2 is essential and plays a role in ciliogenesis and microtubule assembly in Tetrahymena thermophila

BregierC.; Krzemień-OjakL.; W?ogaD.Jerka-DziadoszM.JoachimiakE.BatkoK.FilipiukI.?mietankaU.GaertigJ.FabczakS.FabczakH.

首页> 外文期刊>Journal of Cellular Physiology >Bregier, C.a , Krzemień-Ojak, L.a , W?oga, D.a , Jerka-Dziadosz, M.a , Joachimiak, E.a , Batko, K.a , Filipiuk, I.a , ?mietanka, U.a , Gaertig, J.b , Fabczak, S.a , Fabczak, H.a PHLP2 is essential and plays a role in ciliogenesis and microtubule assembly in Tetrahymena thermophila

【24h】

Bregier, C.a , Krzemień-Ojak, L.a , W?oga, D.a , Jerka-Dziadosz, M.a , Joachimiak, E.a , Batko, K.a , Filipiuk, I.a , ?mietanka, U.a , Gaertig, J.b , Fabczak, S.a , Fabczak, H.a PHLP2 is essential and plays a role in ciliogenesis and microtubule assembly in Tetrahymena thermophila

机译：Bregier, C.a Krzemień-Ojak、洛杉矶、W ?K.a Filipiuk,一、,? mietanka,庞珍庞珍以前Gaertig,有机、Fabczak s.a.、Fabczak H.a PHLP2基本和ciliogenesis和扮演的角色微管装配在四膜虫thermophila

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Recent studies have implicated the phosducin-like protein-2 (PHLP2) in regulation of CCT, a chaperonin whose activity is essential for folding of tubulin and actin. However, the exact molecular function of PHLP2 is unclear. Here we investigate the significance of PHLP2 in a ciliated unicellular model, Tetrahymena thermophila, by deleting its single homolog, Phlp2p. Cells lacking Phlp2p became larger and died within 96h. Overexpressed Phlp2p-HA localized to cilia, basal bodies, and cytosol without an obvious change in the phenotype. Despite similar localization, overexpressed GFP-Phlp2p caused a dominant-negative effect. Cells overproducing GFP-Phlp2p had decreased rates of proliferation, motility and phagocytosis, as compared to wild type cells or cells overproducing a non-tagged Phlp2p. Growing GFP-Phlp2p-overexpressing cells had fewer cilia and, when deciliated, failed to regenerate cilia, indicating defects in cilia assembly. Paclitaxel-treated GFP-Phlp2p cells failed to elongate cilia, indicating a change in the microtubules dynamics. The pattern of ciliary and cytosolic tubulin isoforms on 2D gels differed between wild type and GFP-Phlp2p-overexpressing cells. Thus, in Tetrahymena, PhLP2 is essential and under specific experimental conditions its activity affects tubulin and microtubule-dependent functions including cilia assembly.

机译：最近的研究涉及phosducin-likeprotein-2 (PHLP2)监管的有条件现金转移支付chaperonin的活动是必不可少的微管蛋白和肌动蛋白的折叠。分子PHLP2功能尚不清楚。调查PHLP2的意义在纤毛单细胞模型、四膜虫thermophila,通过删除其单一的相同器官,Phlp2p。死在96 h。本地化的纤毛,基底的身体和细胞溶质没有一个明显的表型变化。尽管类似的本地化,过表达GFP-Phlp2p引起显性负效果。细胞制造出GFP-Phlp2p已经降低了的扩散率,能动性和吞噬作用,与野生型相比,细胞或细胞制造出non-tagged Phlp2p。GFP-Phlp2p-overexpressing细胞纤毛较少当deciliated,未能再生纤毛,表明纤毛装配缺陷。Paclitaxel-treated GFP-Phlp2p细胞没有延长纤毛,指示的变化微管动力学。胞质微管蛋白二维凝胶不同亚型野生型和GFP-Phlp2p-overexpressing之间细胞。并在特定实验条件下活动影响微管蛋白和microtubule-dependent功能包括纤毛组装。

著录项

来源
《Journal of Cellular Physiology》 |2013年第11期|2175-2189|共15页
作者
BregierC.; Krzemień-OjakL.; W?ogaD.Jerka-DziadoszM.JoachimiakE.BatkoK.FilipiukI.?mietankaU.GaertigJ.FabczakS.FabczakH.;
展开▼
作者单位

Department of Cellular Biology, University of Georgia, Athens, Georgia;

Department of Cell Biology, Nencki Institute of Experimental Biology, Warsaw, Poland;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Tubulins; microtubule assembly; Wild typeCytosolexperimental conditionsTetrahymena thermophilaCiliaVolatile Oilscell type;

机译：微管蛋白,微管装配;野外typeCytosolexperimental conditionsTetrahymenathermophilaCiliaVolatile Oilscell类型;

Bregier, C.a , Krzemień-Ojak, L.a , W?oga, D.a , Jerka-Dziadosz, M.a , Joachimiak, E.a , Batko, K.a , Filipiuk, I.a , ?mietanka, U.a , Gaertig, J.b , Fabczak, S.a , Fabczak, H.a PHLP2 is essential and plays a role in ciliogenesis and microtubule assembly in Tetrahymena thermophila

摘要

著录项

相关主题

期刊订阅