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首页> 外文期刊>Journal of Cellular Physiology >Differentiation of human adipose stem cells into neural phenotype by neuroblastoma- or olfactory ensheathing cells-conditioned medium
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Differentiation of human adipose stem cells into neural phenotype by neuroblastoma- or olfactory ensheathing cells-conditioned medium

机译：人类脂肪干细胞的分化神经母细胞瘤或嗅觉神经表型鞘cells-conditioned介质

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Olfactory ensheathing cells (OECs) are known to be capable of continuous neurogenesis throughout lifetime and are a source of multiple trophic factors important in central nervous system regeneration. B104 neuroblastoma cells are recognized to induce differentiation of neural stem cells into oligodendrocyte precursor cells. Therefore, the aim of this study was to verify if conditioned medium (CM) obtained from OECs or B104 cells was capable of inducing differentiation of adipose tissue-derived mesenchymal stem cells (AT-MSCs) to a neuronal phenotype. In order to this goal, immunocytochemical procedures and flow cytometry analysis were used and some neural markers, as nestin, protein gene product 9.5 (PGP 9.5), microtubule-associated protein 2 (MAP2), glial fibrillary acidic protein (GFAP), and neuron cell surface antigen (A2B5) were examined 24h and 7 days after the treatment. The results showed that both OECs- or B104-CM treated AT-MSCs express markers of progenitor and mature neurons (nestin, PGP 9.5 and MAP2) in time-dependent manner, display morphological features resembling neuronal cells, and result negative for GFAP and A2B5, astrocyte and oligodendrocyte markers, respectively. This study demonstrated that AT-MSCs can be influenced by the environment, indicating that these cells can respond to environmental cues also versus a neuronal phenotype.
机译：嗅鞘细胞(近年)是已知的能够连续的神经发生一生,是多种营养的来源因素重要的中枢神经系统再生。公认的诱导分化的神经干细胞为少突细胞前体细胞。因此,本研究的目的是验证从近年或获得的条件培养基(CM)B104细胞诱导的能力分化的脂肪tissue-derived间充质干细胞(AT-MSCs)神经元表现型。采用免疫程序和流式细胞术分析使用和一些神经标记巢蛋白,9.5蛋白基因产物(PGP 9.5),microtubule-associated蛋白2 (MAP2),神经胶质原纤维酸性蛋白(GFAP)和神经元细胞表面抗原(A2B5)检查24小时和7天之后的治疗。近年,或B104-CM AT-MSCs表达治疗祖和成熟神经元的标记(巢蛋白,PGP 9.5和MAP2)以时间的方式,显示形态学特征类似神经细胞,对GFAP和不利结果A2B5、星形胶质细胞和少突细胞标记,分别。AT-MSCs可以受到环境的影响,表明这些细胞可以应对环境因素也与一个神经元表现型。

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