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首页> 外文期刊>Journal of Cellular Physiology >Lipidomic analysis of phospholipids from human mammary epithelial and breast cancer cell lines
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Lipidomic analysis of phospholipids from human mammary epithelial and breast cancer cell lines

机译：从人类Lipidomic磷脂的分析乳腺上皮和乳腺癌细胞系

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Alterations of phospholipid (PL) profiles have been associated to disease and specific lipids may be involved in the onset and evolution of cancer; yet, analysis of PL profiles using mass spectrometry (MS) in breast cancer cells is a novel approach. Previously, we reported a lipidomic analysis of PLs from mouse mammary epithelial and breast cancer cells using off-line thin layer chromatography (TLC)-MS, where several changes in PL profile were found to be associated with the degree of malignancy of cells. In the present study, lipidomic analysis has been extended to human mammary epithelial cells and breast cancer cell lines (MCF10A, T47-D, and MDA-MB-231), using TLC-MS, validated by hydrophilic interaction liquid chromatography-MS. Differences in phosphatidylethanolamine (PE) content relative to total amount of PLs was highest in non-malignant cells while phosphatidic acid was present with highest relative abundance in metastatic cells. In addition, the following differences in PL molecular species associated to cancer phenotype, metastatic potential, and cell morphology were found: higher levels of alkylacyl PCs and phosphatidylinositol (PI; 22:5/18:0) were detected in migratory cells, epithelial cells had less unsaturated fatty acyl chains and shorter aliphatic tails in PE and sphingomyelin classes, while PI (18:0/18:1) was lowest in non-malignant cells compared to cancer cells. To date, information about PL changes in cancer progression is scarce, therefore results presented in this work will be useful as a starting point to define possible PLs with prospective as biomarkers and disclose metabolic pathways with potential for cancer therapy.
机译：改变磷脂(PL)配置文件有关疾病和特定的脂质可能参与发病与进化的癌症;光谱法(MS)在乳腺癌细胞新颖的方法。请从鼠乳腺lipidomic分析使用脱机上皮和乳腺癌细胞薄层色谱法(TLC) - ms,几个PL概要文件被发现是相关的变化恶性肿瘤细胞的程度。本研究,lipidomic分析扩展到人类乳腺上皮细胞乳腺癌细胞系MCF10A T47-D,mda - mb - 231),使用TLC-MS,验证了亲水相互作用液体chromatography-MS。不同磷脂酰乙醇胺(PE)内容相对于总额请最高的良性细胞磷脂酸是目前最高的相对丰度在转移性细胞。PL分子物种相关的差异癌症表型、转移潜能,和细胞形态:发现alkylacyl水平较高个人电脑和磷脂酰肌醇(PI;在迁徙中发现细胞,上皮细胞更少的不饱和脂酰链和短脂肪族的尾巴在PE和鞘磷脂类,而π(18:0/18:1)在罹最低细胞与癌细胞。PL癌症的变化信息发展是稀缺的,因此结果提出了将有用的工作定义可能请与起点潜在生物标志物和披露的代谢途径与潜在的癌症疗法。

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