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首页> 外文期刊>Journal of Cellular Physiology >Functional coupling of TRPC2 cation channels and the calcium-activated anion channels in rat thyroid cells: Implications for iodide homeostasis
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Functional coupling of TRPC2 cation channels and the calcium-activated anion channels in rat thyroid cells: Implications for iodide homeostasis

机译：功能耦合TRPC2阳离子通道calcium-activated阴离子通道的老鼠对碘化甲状腺细胞:影响体内平衡

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The initial step in a synthesis of thyroid hormones is the uptake of iodide from the circulation. Iodide (I-) is transported into thyroid cells via a Na+/I- symporter (NIS), which is electrogenic and thus sensitive to alterations in membrane potential (Vm). I- is then released to the lumen of thyroid follicles where the hormones are synthesised and stored. The mechanisms of I- release to follicle lumen are poorly characterised. Our whole-cell voltage clamp recordings revealed the presence of a Ca2+ activated Cl- current (CaCC) in Fisher rat thyroid cell line 5 (FRTL-5). Transcripts of anoctamin 1 (ANO1) and anoctamin 10 (ANO10), putative molecular constituents of CaCC, were detected. The anion channels underlying CaCC are highly permeable to I-. Both niflumic acid (NFA) and 2-aminoethyl diphenylborinate (2-APB), antagonists of CaCC and transient receptor potential channels, respectively, inhibited CaCC. Canonical transient receptor potential channel 2 (TRPC2) is the only TRPC member present in FRTL-5 cells. The activation rate of CaCC was markedly slower in shTRPC2 knock-down cells, indicating that Ca2+ entry via TRPC2 contributes to CaCC activation. The uptake of iodide was enhanced and the resting Vm was more depolarised in TRPC2 knock-down cells. We suggest that the interplay between TRPC2 and ANO1 may have dual effects on iodide transport, modulating I- release via ANO channels and I- uptake via the Vm sensitive NIS.
机译：第一步合成的甲状腺激素是碘的吸收循环。甲状腺细胞通过Na + / I -同向转运(NIS)改变电致,因此敏感吗在膜电位(Vm)。的甲状腺滤泡腔激素合成和储存。我——释放机制滤泡腔差的特征。夹录音显示Ca2 +的存在激活Cl -电流(中国)费舍尔老鼠甲状腺细胞第5行(FRTL-5)。公认的中国商用飞机有限责任公司的分子组成,检测到。对I -高度渗透。和2-aminoethyl diphenylborinate (2-APB),中国商用飞机有限责任公司和瞬时受体的拮抗剂潜在的渠道,分别抑制中国商用飞机有限责任公司。规范的瞬时受体电位通道2(TRPC2)是唯一出现在FRTL-5 TRPC成员细胞。慢在shTRPC2压倒一切的细胞,指示Ca2 +条目通过TRPC2有助于中国商用飞机有限责任公司激活。休息Vm TRPC2更动摇可拆卸的细胞。TRPC2和ANO1之间可能有双重影响碘化运输,调节通过另我——释放渠道和I -吸收通过NIS Vm敏感。

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