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首页> 外文期刊>Journal of Cellular Physiology >Cerebrospinal fluid miRNA profile in HIV-encephalitis
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Cerebrospinal fluid miRNA profile in HIV-encephalitis

机译：脑脊液microrna的概要文件HIV-encephalitis

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摘要

MicroRNAs are short non-coding RNAs that modulate gene expression by translational repression. Because of their high stability in intracellular as well as extracellular environments, miRNAs have recently emerged as important biomarkers in several human diseases. However, they have not been tested in the cerebrospinal fluid (CSF) of HIV-1 positive individuals. Here, we present results of a study aimed at determining the feasibility of detecting miRNAs in the CSF of HIV-infected individuals with and without encephalitis (HIVE). We also evaluated similarities and differences between CSF and brain tissue miRNAs in the same clinical setting. We utilized a high throughput approach of miRNA detection arrays and identified differentially expressed miRNAs in the frontal cortex of three cases each of HIV+, HIVE, and HIV- controls, and CSF of 10 HIV-positive and 10 HIV-negative individuals. For the CSF samples, the group of HIV+ individuals contained nine cases of HIV-associated neurological disorders (HAND) and, among those, four had HIVE. All the HIV-negative samples had non-viral acute disseminate encephalomyelitis. A total of 66 miRNAs were found differentially regulated in HIV+ compared to HIV- groups. The greatest difference in miRNA expression was observed when four cases of HIVE were compared to five non-HIVE cases, previously normalized with the HIV-negative group. After statistical analyses, 11 miRNAs were fund significantly up-regulated in HIVE. Although more clinical samples should be examined, this work represents the first report of CSF miRNAs in HIV-infection and offers the basis for future investigation. J. Cell. Physiol. ? 2012 Wiley Periodicals, Inc.
机译：小分子核糖核酸短非编码rna调节基因表达的平移镇压。因为他们的高稳定细胞内以及细胞外环境,microrna最近成为重要的生物标志物吗一些人类疾病。被测试的脑脊液(CSF)hiv - 1积极的个人。旨在确定一项研究的结果检测CSF的microrna的可行性感染艾滋病毒的患者脑炎(蜂窝)。异同CSF和脑组织microrna在同一临床设置。我们利用一个高吞吐量的microrna的方式检测数组和识别不同额叶皮层的microrna表达三个例HIV阳性,蜂巢,和HIV -控制,CSF 10阳性和阴性个人。HIV阳性个体包含9例艾滋病毒相关神经障碍(手),其中,四个蜂巢。样品有病毒性急性传播脑脊髓炎。发现不同监管相比,HIV阳性艾滋病毒——组。表达时观察到的四例蜂巢比较五non-HIVE情况下,以前规范化与阴性组。统计分析,11个microrna是基金显著上调蜂巢。临床样本应该检查这个工作代表CSF microrna的第一份报告艾滋病毒感染并提供未来的基础调查。期刊、公司。

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