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首页> 外文期刊>Journal of Cellular Physiology >Caveolae regulate smad signaling as verified by novel imaging and system biology approaches
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Caveolae regulate smad signaling as verified by novel imaging and system biology approaches

机译：小窝调节smad信号验证了新型成像和系统生物学方法

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The contribution of caveolae in Bone Morphogenetic Protein 2 (BMP2) activated Smad signaling was quantified using a system biology approach. BMP2 plays crucial roles during processes such as hematopoiesis, embryogenesis, and skeletal development. BMP2 signaling is tightly regulated on the plasma membrane by its receptors. The localization of BMP receptors in caveolae and endocytosis through clathrin-coated pits are thought to regulate the signaling; however the conclusions in the current literature are inconsistent. Therefore published literature was used to establish a mathematical model that was validated using confocal AFM (atomic force microscopy), confocal microscopy, and sucrose density centrifugation followed by Western blots, and reporter gene assays. The model and experiments confirmed that both caveolae and CCPs regulate the Smad-dependent signaling pathway, however caveolae are centers at the plasma membrane where receptor-ligand interaction is crucial, Smad phosphorylation occurs, and a high degree of Smad signaling is regulated. This demonstrates a role for caveolae that needs to be considered and further studied. J. Cell. Physiol. ? 2012 Wiley Periodicals, Inc.
机译：在骨形成的贡献小窝蛋白2 (BMP2)激活Smad信号使用系统生物学方法的量化。过程,如期间扮演着重要的角色造血作用、胚胎发生和骨骼发展。质膜上的受体。骨形态发生蛋白受体的小窝和本地化内吞作用通过clathrin-coated坑认为控制信号;结论在当前文学不一致的。那是用于建立一个数学模型使用共焦AFM(原子力进行验证显微镜),共焦显微镜和蔗糖密度离心之后,西方的屁股,和报告基因化验。实验证实,小窝和ccp调节Smad-dependent信号通路,然而小窝在等离子体中心膜receptor-ligand交互在哪里重要,Smad发生磷酸化,高程度的Smad信号调控。演示了一个角色需要的小窝考虑和进一步研究。？

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