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首页> 外文期刊>Journal of Cellular Physiology >A pivotal role of bone remodeling in granulocyte colony stimulating factor induced hematopoietic stem/progenitor cells mobilization
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A pivotal role of bone remodeling in granulocyte colony stimulating factor induced hematopoietic stem/progenitor cells mobilization

机译：一个关键的角色在粒细胞骨重塑集落刺激因子诱导造血干细胞/祖细胞动员

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The majority of hematopoietic stem/progenitor cells (HSPCs) reside in bone marrow (BM) surrounded by a specialized environment, which governs HSPC function. Here we investigated the potential role of bone remodeling cells (osteoblasts and osteoclasts) in homeostasis and stress-induced HSPC mobilization. Peripheral blood (PB) and BM in steady/mobilized state were collected from healthy donors undergoing allogeneic transplantation and from mice treated with granulocyte colony stimulating factor (G-CSF), parathyroid hormone (PTH), or receptor activator of nuclear factor kappa-B ligand (RANKL). The number and the functional markers of osteoblasts and osteoclasts were checked by a series of experiments. Our data showed that the number of CD45-Ter119- osteopontin (OPN)+ osteoblasts was significantly reduced from 4,085??135cells/femur on Day 0 to 1,032??55cells/femur on Day 5 in mice (P=0.02) and from 21.38??0.66 on Day 0 to 14.78??0.65 on Day 5 in healthy donors (P0.01). Decrease of osteoblast number leads to reduced level of HSPC mobilization regulators stromal cell-derived factor-1 (SDF-1), stem cell factor (SCF), and OPN. The osteoclast number at bone surface (OC.N/B.s) was significantly increased from 1.53??0.12 on Day 0 to 4.42??0.46 on Day 5 (P0.01) in G-CSF-treated mice and from 0.88??0.20 on Day 0 to 3.24??0.31 on Day 5 (P0.01) in human. Serum TRACP-5b level showed a biphasic trend during G-CSF treatment. The ratio of osteoblasts number per bone surface (OB.N/B.s) to OC.N/B.s was changed after adding PTH plus RANKL during G-CSF treatment. In conclusion, short term G-CSF treatment leads to reduction of osteoblasts and stimulation of osteoclasts, and interrupting bone remodeling balance may contribute to HSPC mobilization. J. Cell. Physiol. ? 2012 Wiley Periodicals, Inc.
机译：大多数的造血干细胞/祖细胞(公司)位于骨髓(BM)专业环境中,包围管理公司的功能。骨重建细胞的潜在作用(成骨细胞和破骨细胞)在体内平衡压力引起的公司动员。稳定的血液(PB)和BM /动员状态从健康的捐赠者进行收集从小鼠同种异体移植和治疗粒细胞集落刺激因子(g - csf)、甲状旁腺激素(甲状旁腺素),或受体kappa-B配体激活的核因素(RANKL)。成骨细胞和破骨细胞是由一个检查一系列的实验。许多CD45-Ter119——骨桥蛋白(OPN) +成骨细胞明显减少4085 ? ?1032 ? ?从21.38 ? 0.66天0到14.78 ? 0.65第五天在健康献血者(术中,0.01)。成骨细胞数量会减少公司的水平动员监管者基质细胞衍生因子- 1 (SDF-1)、干细胞因子(SCF),和OPN。(OC.N /本科)显著增加1.53 ? 0.12天0到4.42 ? 0.46在5天术;0.01)在G-CSF-treated老鼠和0.88 ? 0.20天0到3.24 ? 0.31在5天术;0.01)在人类。两相的趋势在g - csf的治疗。每骨表面成骨细胞数量的比例OC.N / B (OB.N /本科)。甲状旁腺素+ RANKL在g - csf的治疗。结论,短期g - csf治疗导致降低成骨细胞和刺激破骨细胞,阻断骨重塑平衡可能导致公司动员。细胞。

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