Decreased expression of autophagic beclin 1 protein in idiopathic pulmonary fibrosis fibroblasts

RicciA.; CherubiniE.; ScozziD.PietrangeliV.TabbìL.RaffaS.LeoneL.ViscoV.TorrisiM.R.BrunoP.ManciniR.CilibertoG.TerzanoC.MariottaS.

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Decreased expression of autophagic beclin 1 protein in idiopathic pulmonary fibrosis fibroblasts

机译：自噬beclin - 1的表达下降蛋白质在特发性肺纤维化成纤维细胞

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Autophagy is the main cellular pathway for degradation of long-lived proteins and organelles and regulates cell fate in response to stress. Beclin 1 is a key regulator of this process. In some settings autophagy and apoptosis seem to be interconnected. Recent reports indicate that fibroblasts in idiopathic pulmonary fibrosis (IPF) acquire resistance to apoptosis. Here, we examined the expression of beclin 1, and of the anti apoptotic protein Bcl-2 in human IPF fibroblasts using immunohistochemistry and molecular biology in bioptic sections, in primary cultures of fibroblasts taken from patients with IPF and in fibroblast cell lines. Expression of beclin 1 in fibroblasts from IPF was down-regulated in comparison with fibroblasts from normal lungs while the anti-apoptotic protein Bcl-2 expression was over-expressed. Treatment of fibroblast cell cultures with cisplatin induced a significant increase in beclin 1 and caspase 3 protein levels but a reduction in Bcl-2 expression. These observations were confirmed by the analysis of acid compartments and transmission electron microscopy. Our results demonstrate a modified expression of the apoptotic beclin 1 Bcl-2 proteins in human IPF fibroblasts suggesting the existence of an autophagy/apoptosis system dysfunction.

机译：细胞自噬是主要的途径退化的长寿蛋白质和细胞器在对压力的反应,调节细胞命运。Beclin 1是这个过程的关键调节器。自噬和凋亡似乎有些设置相互联系的。成纤维细胞在特发性肺纤维化(IPF)获得抗细胞凋亡。检查beclin - 1的表达,抗凋亡蛋白bcl - 2在人类IPF成纤维细胞使用免疫组织化学和分子生物学在活组织检查的部分,在初级成纤维细胞来自患者的文化IPF和纤维母细胞细胞系。beclin 1 IPF的成纤维细胞抑制与成纤维细胞相比从正常肺而抗凋亡bcl - 2蛋白的表达增加。治疗纤维母细胞的细胞培养顺铂诱导显著增加但beclin 1和半胱天冬酶3蛋白水平bcl - 2表达的减少。经酸的分析吗隔间和透射电子显微镜。bcl - 2的表达凋亡beclin 1蛋白质在人类IPF成纤维细胞暗示存在自噬/凋亡系统功能障碍。

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《Journal of Cellular Physiology》 |2013年第7期|1516-1524|共9页
作者
RicciA.; CherubiniE.; ScozziD.PietrangeliV.TabbìL.RaffaS.LeoneL.ViscoV.TorrisiM.R.BrunoP.ManciniR.CilibertoG.TerzanoC.MariottaS.;
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作者单位

Dipartimento di Scienze Cardiovascolari, Respiratorie, Nefrologiche e Geriatriche, Università La;

Dipartimento di Medicina Sperimentale e Clinica, Università Magna Grecia, Catanzaro, Italy, IRCCS;

Dipartimento di Medicina Clinica e Molecolare, Università La Sapienza, Azienda Ospedaliera Sant;

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正文语种英语
中图分类细胞生物学;
关键词
primary culture; Autophagy; Fibroblastbcl-2 GenesBeclin-1Cell fateIdiopathic Pulmonary Fibrosis;

机译：主要文化;自噬;Fibroblastbcl-2纤维化;

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Decreased expression of autophagic beclin 1 protein in idiopathic pulmonary fibrosis fibroblasts

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