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首页> 外文期刊>Journal of Cellular Physiology >Agonist-activated Ca 2+ influx and Ca 2+-dependent Cl - channels in Xenopus ovarian follicular cells: Functional heterogeneity within the cell monolayer
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Agonist-activated Ca 2+ influx and Ca 2+-dependent Cl - channels in Xenopus ovarian follicular cells: Functional heterogeneity within the cell monolayer

机译：Agonist-activated Ca 2 +涌入和Ca 2 +端依赖Cl -渠道在非洲爪蟾蜍卵巢滤泡细胞:细胞内的功能异质性单层

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摘要

Xenopus follicles are endowed with specific receptors for ATP, ACh, and AII, transmitters proposed as follicular modulators of gamete growth and maturation in several species. Here, we studied ion-current responses elicited by stimulation of these receptors and their activation mechanisms using the voltage-clamp technique. All agonists elicited Cl - currents that depended on coupling between oocyte and follicular cells and on an increase in intracellular Ca 2+ concentration ([Ca 2+] i), but they differed in their activation mechanisms and in the localization of the molecules involved. Both ATP and ACh generated fast Cl - (F Cl) currents, while AII activated an oscillatory response; a robust Ca 2+ influx linked specifically to F Cl activation elicited an inward current (I iw,Ca) which was carried mainly by Cl - ions, through channels with a sequence of permeability of SCN -I -Br -Cl -. Like F Cl, I iw,Ca was not dependent on oocyte [Ca 2+] i; instead both were eliminated by preventing [Ca 2+] i increase in the follicular cells, and also by U73122 and 2-APB, drugs that inhibit the phospolipase C (PLC) pathway. The results indicated that F Cl and I iw,Ca were produced by the expected, PLC-stimulated Ca 2+-release and Ca 2+-influx, respectively, and by the opening of I Cl(Ca) channels located in the follicular cells. Given their pharmacological characteristics and behavior in conditions of divalent cation deprivation, Ca 2+-influx appeared to be driven through store-operated, calcium-like channels. The AII response, which is also known to require PLC activation, did not activate I iw,Ca and was strictly dependent on oocyte [Ca 2+] i increase; thus, ATP and ACh receptors seem to be expressed in a population of follicular cells different from that expressing AII receptors, which were coupled to the oocyte through distinct gap-junction channels. J. Cell. Physiol. 227: 3457-3470, 2012.
机译：非洲爪蟾蜍毛囊被赋予特定的对ATP受体,靠,暗生,发射器提出了滤泡调节器的配子在几个物种的生长和成熟。我们研究了离子电流所引起的反应这些受体及其刺激使用电压钳位激活机制技术。这取决于卵母细胞和之间的耦合滤泡细胞的增加约2 +糜烂(intracellular [Ca 2 +]),但他们在激活机制不同和本地化的分子参与。Cl)电流,激活一个振荡暗生反应;具体到F Cl激活引起的内向电流(我iw, Ca)是主要通过Cl -离子通道的序列渗透率的SCN→我→Br→Cl -。喜欢F Cl,信息战,并不依赖于卵母细胞(Ca 2 +)我;防止(Ca 2 +)我增加滤泡细胞,还有U73122 2-APB,药物抑制phospolipase C (PLC)通路。结果表明,F Cl iw, Ca产生的预期,PLC-stimulated Ca2 +释放和Ca 2 +涌入,分别开幕式我Cl (Ca)位于渠道滤泡细胞。特点和行为的条件二价阳离子剥夺,Ca 2 +涌入似乎是通过门店,calcium-like频道。也需要PLC激活,没有激活我的信息战,Ca,并严格依赖卵母细胞(Ca 2 +)我增加;受体似乎表现在人口滤泡细胞不同于表达受体,耦合到卵母细胞通过不同的缝隙连接通道。杂志。227:3457 - 3470年,2012年。

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