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首页> 外文期刊>Journal of Cellular Physiology >Lysyl hydroxylase 3 is secreted from cells by two pathways.
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Lysyl hydroxylase 3 is secreted from cells by two pathways.

机译：由两个Lysyl羟化酶3分泌细胞通路。

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摘要

Lysyl hydroxylase 3 (LH3) is a post-translational modification enzyme with lysyl hydroxylase (LH), collagen galactosyltransferase (GT), and glucosyltransferase (GGT) activities. The active sites responsible for LH and GT/GGT activities of LH3 are localized separately in the carboxy- and the amino-terminal parts of the molecule, respectively. LH3 is found both intracellularly in the ER, as well as extracellularly in serum, the extracellular space and on cell surfaces, and is the only secreted LH isoform. In order to determine whether the activities of LH3 play a role in the secretion, we created various LH3 and mutant expression constructs and over-expressed the proteins in COS-7 and HT-1080 cells. Our data indicate that while the LH active site mediates retention of LH3 in the ER, the GGT active site is required for the secretion of LH3 into the extracellular space. Moreover, Brefeldin A treatment and cholesterol depletion of the cells revealed that the secretion of LH3 from the ER to the extracellular space occurs via two secretory pathways, which generate two glycoforms. LH3 molecules found in the cell medium are secreted through the Golgi complex, and the secretion is dependent on LH3 glycosyltransferase activity. LH3 found on the cell surface bypasses the Golgi complex.
机译：Lysyl羟化酶3 (LH3)是一种翻译后修饰酶与lysyl羟化酶(LH),胶原蛋白半乳糖基转移酶(GT)葡糖基转移酶(GGT)活动。网站负责LH和GT / GGT的活动羧基和LH3分别本地化伴的部分分子,分别。呃,以及血清中细胞外,细胞外空间和细胞表面是唯一的分泌LH同种型。确定是否LH3发挥的活动在分泌中的作用,我们创建了各种LH3和突变表达结构和过度COS-7和ht - 1080细胞的蛋白质。表明虽然LH活性部位调和保留的LH3呃,GGT活性部位需要的分泌LH3到吗细胞外空间。治疗和细胞的胆固醇耗竭透露,LH3从ER的分泌通过两个分泌细胞外空间发生途径,产生两个改变。分子在细胞中分泌通过高尔基氏复合体,并分泌依赖LH3糖基转移酶的活动。LH3发现在细胞表面绕过了高尔基复杂。

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