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首页> 外文期刊>Journal of Cellular Physiology >Development and evaluation of a trehalose-contained solution formula to preserve hUC-MSCs at 4°C
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Development and evaluation of a trehalose-contained solution formula to preserve hUC-MSCs at 4°C

机译：开发和评估的trehalose-contained公式保护的解决方案

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摘要

Human mesenchymal stem cells (hMSCs) hold great promise in cell therapy and regenerative medicine. Various preclinical and clinical trials have been carried out to illustrate the therapeutic potential of these cells. However, one major challenge for manufacturing clinical grade hMSCs is the requisition of current good manufacturing practice (cGMP) grade practices in cell isolation, processing, storage, and distribution. Development of non-toxic and animal serum-free preservation medium is critical for storage and distribution of mesenchymal stem cells (MSCs). In this study, we developed a solution formula that could preserve MSCs at 4°C for up to 3 weeks. In the solution, trehalose is a key ingredient for maintaining survival of MSCs. Among the concentrations investigated, 40mM trehalose showed the best outcome with the viability maintained more than 92.7±1.5% for 7 days. Cells preserved in the solution formula for 3 weeks still remained about 70% viability, and produced results similar to those of freshly harvested hMSCs in terms of growth kinetics, expression profile of cell surface antigens, and differentiation potential. In summary, storage of MSCs in the medium makes it far easier for transporting the cells from processing units to clinical sites.
机译：人类间充质干细胞(hMSCs)大承诺在细胞治疗和再生医学。进行了说明了吗这些细胞的治疗潜力。一个主要的制造业的临床挑战年级hMSCs当前良好的征用制造业(cGMP)年级实践练习细胞分离、加工、存储和分布。血清保护中是至关重要的存储和间充质干细胞的分布细胞(msc)。解公式,可以保存msc在4°C3周。维持生存的一个重要因素msc。海藻糖的最好的结果生存能力保持超过92.7±1.5% 7天。3周仍约70%的可行性刚得到类似的结果收获hMSCs生长动力学,细胞表面抗原的表达谱,分化的潜能。msc在中使它更容易运输细胞从处理单元临床基地。

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