...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Functional cGMP-gated channels in cerebellar granule cells
【24h】

Functional cGMP-gated channels in cerebellar granule cells

机译：在小脑功能cGMP-gated通道颗粒细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cyclic nucleotide-gated channels (CNGCs) are important transducers of external signals in sensory processes. These channels are ubiquitously expressed in a variety of neurons, and are necessary to transduce signals for growth cone guidance and plasticity. Here, we demonstrate that the CNGC subunits (CNGA1 and CNGB1, presumably the 1b isoform) are expressed in rat cerebellar granule cells and that they combine to form functional channels. The expression of the mRNAs that encode these proteins is maximal after 7 days in cell culture, when the channels are expressed at synapses and co-localize with the synaptic marker synapsin I. These ligand-gated channels are functional and can be blocked by Mg 2+ or L-cis-diltiazem. Moreover, channel opening in response to increases in intracellular cGMP results in Ca 2+ entry into the cell. Chronic blockade (96h) of these channels with L-cis-diltiazem significantly decreases the number of functional boutons, as determined by their capacity to load and unload the styryl dye FM1-43 when stimulated. Moreover, the unloading kinetics is modified from a biphasic to a monophasic profile in a subset of synaptic boutons. These channels are also expressed in early developmental stages, both in the soma and in emerging processes, and CNGA1 can be detected in growth cones. Pharmacological blockade of these channels with L-cis-diltiazem causes an overall change in growth cone morphology, impairing the formation of lamellipodia between filopodia and increasing the number of filopodia.
机译：循环nucleotide-gated渠道(中国)重要的外部信号的传感器感觉过程。广泛表达在不同的神经元,增长和转导信号是必要的锥指导和可塑性。证明中国的子单元(CNGA1和CNGB1,大概1 b同种型)表示在大鼠小脑颗粒细胞,他们结合形成功能的通道。mrna的表达编码蛋白质是最大的在细胞培养7天之后,当突触和表达的渠道硝唑与突触标记synapsin我。这些ligand-gated渠道功能和可以被Mg 2 +或L-cis-diltiazem。此外,通道开放以应对增加细胞内cGMP Ca 2 +进入细胞。这些通道L-cis-diltiazem显著减少功能剑头的数量,加载和卸载取决于他们的能力苯乙烯基染料FM1-43刺激的时候。从一个卸货动力学修改两相的单相概要文件的一个子集突触的剑头。表示在早期发展阶段,无论是soma和新兴流程和CNGA1即可被发现在生长锥。这些渠道与L-cis-diltiazem的封锁导致整体生长锥的变化形态、损伤的形成板状伪足丝状伪足和增加之间的关系丝状伪足的数量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号