Arecoline-induced death of human leukemia K562 cells is associated with surface up-modulation of TNFR2

ChenY.-J.; ChangL.-S.

首页> 外文期刊>Journal of Cellular Physiology >Arecoline-induced death of human leukemia K562 cells is associated with surface up-modulation of TNFR2

【24h】

Arecoline-induced death of human leukemia K562 cells is associated with surface up-modulation of TNFR2

机译：人类白血病K562 Arecoline-induced死亡细胞与表面up-modulation相关联TNFR2

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The goal of the present study is to explore the contribution of tumor necrosis factor-α (TNFα)-related pathway to the cytotoxicity of arecoline on human leukemia K562 cells. Arecoline treatment induced death of K562 cells and increased surface expression of TNFα, TNFR1, and TNFR2. Unlike that of TNFR1 mRNA, transcriptional levels of TNFα and TNFR2 mRNA increased in arecoline-treated cells. Moreover, arecoline-induced down-regulation of ADAM17 maturation was involved in surface up-modulation of TNFR1, TNFR2, and TNFα. Arecoline-elicited increase in intracellular Ca 2+ concentration was responsible for JNK/c-Jun pathway activation and ERK inactivation. Abolition of JNK/c-Jun pathway suppressed arecoline-induced increase in transcriptional level of TNFα and TNFR2 mRNA. TNFα and TNFR2 promoter luciferase activity and chromatin immunoprecipitating analyses revealed that c-Jun increasingly bound with TNFα and TNFR2 promoter upon arecoline treatment. Over-expression of constitutively active MEK1 abolished the effect of arecoline on suppressing ADAM17 maturation. Pretreatment with TNFR2 antibody abrogated arecoline-induced increased susceptibility of K562 cells for the cytotoxicity of TNFα and arecoline-induced cell death. Taken together, our data suggest that up-modulation of TNFR2 surface expression is associated with arecoline-induced death of K562 cells.

机译：本研究的目的是探索肿瘤坏死因子-α的贡献(肿瘤坏死因子α)-相关通路的细胞毒性槟榔碱对人类白血病K562细胞。治疗诱导K562细胞的死亡增加表面的肿瘤坏死因子α的表达,TNFR1,TNFR2。肿瘤坏死因子α和TNFR2 mRNA水平增加arecoline-treated细胞。arecoline-induced ADAM17下调成熟up-modulation参与表面TNFR1 TNFR2,肿瘤坏死因子α。增加细胞内钙浓度2 +负责物/ c-Jun途径激活ERK失活。抑制arecoline-induced增加肿瘤坏死因子α和TNFR2 mRNA转录水平。肿瘤坏死因子α和TNFR2启动子荧光素酶活性染色质免疫沉淀反应分析显示与肿瘤坏死因子α和TNFR2 c-Jun越来越束缚启动子在槟榔碱治疗。表达的MEK1持续活跃废除了槟榔碱对抑制的影响ADAM17成熟。抗体废除arecoline-induced增加易感性K562细胞的细胞毒性肿瘤坏死因子α和arecoline-induced细胞死亡。在一起,我们的数据表明,up-modulationTNFR2表面表达有关arecoline-induced K562细胞的死亡。

著录项

来源
《Journal of Cellular Physiology》 |2012年第5期|2240-2251|共12页
作者
ChenY.-J.; ChangL.-S.;
展开▼
作者单位

Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
K562 Cells; Cell Death; Tumor Necrosis Factor Receptor Type IIArecolineTumor Necrosis Factor Receptor 1AHUMAN LEUKAEMIA CELLTumor Necrosis Factor-alphaK-562 cell line;

机译：K562细胞;细胞死亡;肿瘤坏死因子IIArecolineTumor坏死因子受体类型受体1人际关系白血病CELLTumor坏死因子- alphak - 562细胞系;

相似文献

外文文献
中文文献

1. Inhibition of Erythroid Differentiation of Human Leukemia K562 Cells by N-acetylcysteine and Ascorbic Acid through Downregulation of ROS [J] . YU Chun Hong, JIANG Liang, WANG Ying, 生物医学与环境科学（英文版） . 2018,第003期
2. Cell death-independent activities of the death receptors CD95, TRAILR1, and TRAILR2 [J] . The FEBS journal . 2017,第8期

机译：Cell Death-Death-Death受体的活动活动CD95，Trailr1和Trailr2
3. Adu; Aged;; Aged, 80 and over;; Cadherins;; Carcinoma, Squamous Cell;; Cell Nucleus;; Cytoplasm;; Female;; Humans;; Laryngeal Neoplasms;; Male;; Middle Aged;; Neoplasm Staging;; Protein-Serine-Threonine Kinases;; Proto-Oncogene Proteins c-akt;; Retrospective Studies;; beta Catenin;; *成年人;; 老年人;; 老年人, 80以上;; 钙粘着糖蛋白类;; 癌, 鳞状细胞;; 细胞核;; 细胞质;; 喉肿瘤;; 肿瘤分期;; 蛋白质丝氨酸苏氨酸激酶;; 回顾性研究 [J] . Acta Anaesthesiologica Scandinavica . 2009,第10期

机译：Adu; Aged;; Aged, 80 and over;; Cadherins;; Carcinoma, Squamous Cell;; Cell Nucleus;; Cytoplasm;; Female;; Humans;; Laryngeal Neoplasms;; Male;; Middle Aged;; Neoplasm Staging;; Protein-Serine-Threonine Kinases;; Proto-Oncogene Proteins c-akt;; Retrospective Studies;; beta Catenin;; *成年人;; 老年人;; 老年人, 80以上;; 钙粘着糖蛋白类;; 癌, 鳞状细胞;; 细胞核;; 细胞质;; 喉肿瘤;; 肿瘤分期;; 蛋白质丝氨酸苏氨酸激酶;; 回顾性研究
4. Knockout of Arabidopsis ACCELERATED-CELL-DEATH11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense [O] . Peter Brodersen, Morten Petersen, Helen M. Pike, 2002

机译：编码鞘氨醇转移蛋白的拟南芥ACCELERATED-CELL-DEATH11基因的敲除导致程序性细胞死亡和防御的激活
5. Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic Activity Contributes to Interferon-Gamma Induced Apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells [O] . Chung, Ting Wen, Tan, Kok-Tong, Chan, Hong-Lin, 2015

机译：Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic activity Contributes to Interferon-Gamma Induced apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells

Arecoline-induced death of human leukemia K562 cells is associated with surface up-modulation of TNFR2

摘要

著录项

相似文献

相关主题

期刊订阅