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首页> 外文期刊>Journal of Cellular Physiology >Gradients of natriuretic peptide precursor A (NPPA) in oviduct and of natriuretic peptide receptor 1 (NPR1) in spermatozoon are involved in mouse sperm chemotaxis and fertilization
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Gradients of natriuretic peptide precursor A (NPPA) in oviduct and of natriuretic peptide receptor 1 (NPR1) in spermatozoon are involved in mouse sperm chemotaxis and fertilization

机译：利钠肽前体的梯度(NPPA)输卵管和利钠肽在精子受体1 (NPR1)参与老鼠精子的趋化性和受精

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摘要

Selective spermatozoa movement from storage of the oviduct to fertilization site is suggested to be a result of chemotaxis. In the present study, Natriuretic peptide precursor A (NPPA) induced sperm chemotaxis in capillaries and enhanced intracellular Ca 2+ level, both of which could be blocked by the Natriuretic Peptide Receptor 1 (NPR1) inhibitor anantin and the cGMP-dependent protein kinase (PKG) inhibitors, KT5823 and Rp-8-Br-PET-cGMPS. NPPA also increased spermatozoa kinetic parameters of VAP, VSL, LIN, STR, and BCF. Only 2.0% of positive staining for NPR1 was detected in fresh spermatozoa. The positive rate was increased in capacitated spermatozoa (20.5%), and further increased in spermatozoa of NPPA treatment (70.2%). Nppa mRNA level in the ampullae was significantly higher compared with that in isthmus and uterotubal junction, and NPPA protein had an ascending gradient (AG) from the uterotubal junction to ampullae in gonadotropin-treated mice. NPPA induced sperm chemotaxis in diestrus oviducts without a NPPA gradient, and sperm chemotaxis occurred in the oviducts of gonadotropin-treated mice. These effects were inhibited by anantin. Meanwhile, sperm chemotaxis also occurred in unilateral ovariectomized oviducts of gonadotropin-treated mice, in which the possible effect of follicular fluid and oocyte-cumulus mass were eliminated when ovulation occurs. Furthermore, anantin significantly decreased the rate of fertilization in a dose-dependent manner (0.1μM, 57.1%; 1μM, 33.8%) compared with control (78.5%). These results suggest that a NPPA gradient originating in the oviduct induces sperm chemotaxis by binding to its receptor NPR1 and then activating PKG pathway, and plays a physiological role in fertilization.
机译：有选择性的精子运动的存储输卵管受精网站建议趋化作用的结果。利钠肽前体(NPPA)诱导在毛细血管和增强精子的趋化作用细胞内钙2 +级,这两个可能被利钠肽受体1(NPR1)抑制剂anantin cGMP-dependent蛋白激酶(PKG)抑制剂,KT5823和Rp-8-Br-PET-cGMPS。林:VAP精子运动参数的,,STR和供应量。NPR1中检测出新鲜的精子。在生产积极率增加精子(20.5%),并进一步增加精子NPPA治疗(70.2%)。壶腹明显更高的水平相比之下,在地峡和子宫输卵管结,NPPA蛋白质有一个提升梯度(AG)从子宫输卵管结壶腹gonadotropin-treated老鼠。间情期输卵管精子诱导趋化作用没有NPPA梯度,精子的趋化作用发生在输卵管里gonadotropin-treated老鼠。与此同时,精子趋化作用也发生在单方面切除卵巢的输卵管gonadotropin-treated老鼠,可能的卵泡液和oocyte-cumulus的效果质量排卵发生时就被淘汰了。此外,anantin显著下降受精剂量依赖性的方式(0.1μM, 57.1%;(78.5%)。梯度起源于输卵管引起精子绑定到其受体NPR1和趋化性然后激活包裹途径,发挥了在受精的生理作用。

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