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首页> 外文期刊>Journal of Cellular Physiology >Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin
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Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin

机译：Acetylation-dependent核安排和招聘UV-damaged BMI1蛋白质染色质

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摘要

Polycomb group (PcG) proteins, organized into Polycomb bodies, are important regulatory components of epigenetic processes involved in the heritable transcriptional repression of target genes. Here, we asked whether acetylation can influence the nuclear arrangement and function of the BMI1 protein, a core component of the Polycomb group complex, PRC1. We used time-lapse confocal microscopy, micro-irradiation by UV laser (355nm) and GFP technology to study the dynamics and function of the BMI1 protein. We observed that BMI1 was recruited to UV-damaged chromatin simultaneously with decreased lysine acetylation, followed by the recruitment of heterochromatin protein HP1β to micro-irradiated regions. Pronounced recruitment of BMI1 was rapid, with half-time τ=15sec; thus, BMI1 is likely involved in the initiation step leading to the recognition of UV-damaged sites. Histone hyperacetylation, stimulated by HDAC inhibitor TSA, suppression of transcription by actinomycin D, and ATP-depletion prevented increased accumulation of BMI1 to γH2AX-positive irradiated chromatin. Moreover, BMI1 had slight ability to recognize spontaneously occurring DNA breaks caused by other pathophysiological processes. Taken together, our data indicate that the dynamics of recognition of UV-damaged chromatin, and the nuclear arrangement of BMI1 protein can be influenced by acetylation and occur as an early event prior to the recruitment of HPβ to UV-irradiated chromatin.
机译：Polycomb集团(PcG)蛋白质,组织成Polycomb身体,监管很重要组件的表观遗传过程遗传转录镇压目标基因。会影响核安排吗BMI1蛋白质的功能,一个核心组成部分Polycomb组复杂,PRC1。延时的共焦显微镜,micro-irradiation通过紫外激光(355海里)和GFP技术研究BMI1动力学和功能的蛋白质。观察到BMI1 UV-damaged招募染色质同时减少赖氨酸乙酰化作用,其次是招聘的异染色质HP1蛋白βmicro-irradiated地区。快速,半场τ= 15秒;可能参与了导致起始步骤UV-damaged网站的识别。HDAC抑制剂hyperacetylation,刺激美国运输安全管理局,由放线菌素抑制转录D, ATP-depletion阻止增加积累的BMI1γH2AX-positive辐照染色质。识别自发发生DNA断裂由其他病理生理过程引起的。综上所述,我们的数据表明承认UV-damaged染色质动力学,和BMI1蛋白质的核协议由乙酰化作用和发生影响早期的事件之前,惠普β的招聘紫外线照射过的染色质。

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