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首页> 外文期刊>Journal of Cellular Physiology >Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway
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Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway

机译：玉米烯酮诱导细胞凋亡和坏死通过caspase-3——猪颗粒细胞caspase-9-dependent线粒体信号通路

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摘要

Zearalenone is a mycotoxin produced mainly by Fusarium. There are numerous incidences of mycotoxicosis in laboratory and domestic animals, especially in pigs. However, little is known about molecular mechanisms of zearalenone toxicity. Granulosa cells are the maximal cell population in follicles, and they play an essential role in the development and maturation of follicles. The objective of this study was to explore the effect of zearalenone at high concentrations on proliferation and apoptosis of porcine granulosa cells and uncover signaling pathway underlying the cytotoxicity of zearalenone. We found that zearalenone reduced the proliferation of porcine granulosa cells in a dose-dependent manner as shown by the MTT assay and zearalenone resulted in an obvious apoptosis and necrosis in porcine granulosa cells as determined by the TUNEL analysis and flow cytometry. In addition, TUNEL assay with caspase inhibitors showed that zearalenone triggered a caspase-3- and caspase-9-dependent apoptotic process in porcine granulosa cells. Fluorescence spectrophotometer displayed that zearalenone led to a loss of mitochondrial transmembrane potential of porcine granulosa cells but enhanced reactive oxygen species (ROS) levels of the cells. Notably, Western blots revealed that caspase-3 and caspase-9 were activated by zearalenone in porcine granulosa cells. Collectively, our results suggest that zearalenone induces apoptosis and necrosis of porcine granulosa cells in a dose-dependent manner via a caspase-3- and caspase-9-dependent mitochondrial pathway. This study thus offers a novel insight into molecular mechanisms by which zearalenone has adverse cytotoxicity on reproduction.
机译：玉米烯酮是一种霉菌毒素主要是由生产镰刀菌素。霉菌毒素中毒病在实验室和家畜,特别是在猪。玉米烯酮的分子机制毒性。在毛囊人口,他们发挥重要作用的发展和成熟毛囊。探索玉米烯酮在高的效果浓度细胞增殖和细胞凋亡猪颗粒细胞,发现信号路径潜在的细胞毒性玉米烯酮。猪颗粒细胞的扩散MTT试验剂量依赖性的方式如图所示和玉米烯酮导致一个明显的细胞凋亡在猪颗粒细胞和坏死由TUNEL分析和流血细胞计数。抑制剂表明玉米烯酮引发了caspase-3——caspase-9-dependent凋亡猪颗粒细胞的过程。分光光度计显示玉米烯酮线粒体跨膜的丧失猪颗粒细胞,但增强的潜力活性氧(ROS)的水平细胞。caspase-3和caspase-9被激活玉米烯酮在猪颗粒细胞。总的来说,我们的研究结果表明,玉米烯酮诱发的细胞凋亡和坏死猪颗粒细胞存在剂量依赖的相关性通过caspase-3——caspase-9-dependent方式线粒体途径。小说洞察分子机制玉米烯酮不良细胞毒性繁殖。

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