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首页> 外文期刊>Journal of Cellular Physiology >Peripheral nerve pericytes modify the blood-nerve barrier function and tight junctional molecules through the secretion of various soluble factors.
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Peripheral nerve pericytes modify the blood-nerve barrier function and tight junctional molecules through the secretion of various soluble factors.

机译：周围神经的周修改2屏障功能和紧密联接的分子通过各种可溶性因子的分泌。

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摘要

The objectives of this study were to establish pure blood-nerve barrier (BNB) and blood-brain barrier (BBB)-derived pericyte cell lines of human origin and to investigate their unique properties as barrier-forming cells. Brain and peripheral nerve pericyte cell lines were established via transfection with retrovirus vectors incorporating human temperature-sensitive SV40 T antigen (tsA58) and telomerase. These cell lines expressed several pericyte markers such as alpha-smooth muscle actin, NG2, platelet-derived growth factor receptor beta, whereas they did not express endothelial cell markers such as vWF and PECAM. In addition, the inulin clearance was significantly lowered in peripheral nerve microvascular endothelial cells (PnMECs) through the up-regulation of claudin-5 by soluble factors released from brain or peripheral nerve pericytes. In particular, bFGF secreted from peripheral nerve pericytes strengthened the barrier function of the BNB by increasing the expression of claudin-5. Peripheral nerve pericytes may regulate the barrier function of the BNB, because the BNB does not contain cells equivalent to astrocytes which regulate the BBB function. Furthermore, these cell lines expressed several neurotrophic factors such as NGF, BDNF, and GDNF. The secretion of these growth factors from peripheral nerve pericytes might facilitate axonal regeneration in peripheral neuropathy. Investigation of the characteristics of peripheral nerve pericytes may provide novel strategies for modifying BNB functions and promoting peripheral nerve regeneration.
机译：本研究的目标是建立纯2屏障(BNB)和血脑障碍(BBB)派生的周皮细胞细胞系人类起源和调查他们独特的属性barrier-forming细胞。周围神经外膜细胞细胞系通过建立与逆转录病毒转染向量将人类热敏SV40 T抗原(tsA58)和端粒酶。行表示几周皮细胞标记等alpha-smooth肌肉肌动蛋白,喜欢的《忍者外传2》,血小板源生长因子受体β,而他们没有表达内皮细胞标记物如vWF和PECAM。在周围神经显著降低微血管内皮细胞(PnMECs)claudin-5老年病的可溶性因子从大脑释放或周围神经周。周围神经的周加强了BNB通过增加的屏障功能claudin-5的表情。周可以调节的屏障功能BNB,因为BNB不包含细胞相当于调节BBB的星形胶质细胞函数。多种神经营养因子如神经生长因子、脑源性神经营养因子,和GDNF。从周围神经的周会方便在周围神经病变轴突再生。调查的特点周围神经的周可能提供的小说修改BNB功能和策略促进周围神经再生。

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