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首页> 外文期刊>Journal of Cellular Physiology >Evidence of specific characteristics and osteogenic potentiality in bone cells from tibia.
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Evidence of specific characteristics and osteogenic potentiality in bone cells from tibia.

机译：的具体特点和证据在从胫骨骨细胞成骨的潜力。

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Human bone cells used for in vitro studies are mainly derived from bone marrow (BM) or trabecular bone (TB). There are no specific markers or procedures for isolation and growth of these cells. To validate the potentiality of these cells, we isolated human mesenchymal stromal cells (MSCs) and osteoblasts (OBs) from the tibial plateau of the same subject, grown in two different media (alpha-MEM and DMEM/F12) and analyzed for cell growth, proliferation, phenotype and osteogenic potential. We found that OBs grew well in both media tested, but MSCs were able to grow only in alpha-MEM medium. OBs in DMEM/F12 showed reduced proliferation capability and expressed a low level of alkaline phosphatase (AP), RUNX-2, osteocalcin (OC), bone sialoprotein (BSP), collagen type I (Col.I) compared with OBs in alpha-MEM but high level of collagen type XV (Col.XV). Compared with MSCs in alpha-MEM, OBs have an increased ability to proliferate and express more OC and BSP at molecular level but less AP, RUNX-2 and Col.I than MSCs. Time-course experiments to analyze the osteogenic potential of these cells showed that OBs were more efficient than MSCs. However, these cells obtained from tibial plateau showed a different trend of AP, OC and Col.I osteogenic markers compared to control MSCs from the iliac crest. This study shows that bone-adherent OBs grown in alpha-MEM medium are more efficient for osteogenic differentiation than BM MSCs and contribute to defining their phenotypic and functional characteristics, so providing a rationale for their use in bone tissue engineering or therapeutic purposes.
机译：人类骨细胞用于体外研究主要来源于骨髓(BM)或骨小梁(结核病)。标记或程序隔离和增长这些细胞。这些细胞,我们孤立的人类间充质基质细胞(msc)和成骨细胞(观察)胫骨高原相同的主题,在增长两种不同的媒体(alpha-MEM和DMEM / F12)和分析细胞生长、增殖表型和成骨的潜力。观察生长良好,在这两种媒体测试,但msc能只生长在alpha-MEM媒介。DMEM / F12显示减少扩散能力和碱性磷酸酶表达低水平(美联社)、RUNX-2、骨钙素(OC),骨涎蛋白(BSP),胶原蛋白I型(Col.I)与观察alpha-MEM但高水平的胶原蛋白类型十五(Col.XV)。增殖能力和增加吗但表达更多OC、BSP分子水平更少的美联社,RUNX-2和Col.I msc。实验分析成骨的潜力这些细胞显示奥林匹克广播服务公司效率比msc。从胫骨平台显示不同美联社的趋势,OC和Col.I成骨的标记从髂嵴相比控制msc。这项研究表明,bone-adherent奥林匹克广播服务公司在增长alpha-MEM媒介更有效比BM msc及成骨分化为定义他们的表型和做出贡献功能特性,所以提供一个理由在骨组织中使用工程或治疗的目的。

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