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首页> 外文期刊>Journal of Cellular Physiology >Decorin and chondroitin-6 sulfate inhibit B16V melanoma cell migration and invasion by cellular acidification.
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Decorin and chondroitin-6 sulfate inhibit B16V melanoma cell migration and invasion by cellular acidification.

机译：Decorin和硫酸chondroitin-6抑制B16V黑色素瘤细胞迁移和入侵细胞酸化。

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摘要

In vivo, cells are embedded in an environment generated and maintained by multiple cell-cell and cell-matrix interactions. While transiting the dermis metastasizing melanoma cells interact with the extracellular matrix (ECM) and fibroblasts. To study the roles of ECM components and fibroblasts in melanoma (B16V) cell migration and invasion, we established a co-culture system consisting of fibroblasts, their collagen-rich matrix and B16V cells. The crosstalk between B16V cells and fibroblasts was indicated by a clear increase in release and activity of matrix-metallo-protease-2. Time-lapse microscopy revealed that in B16V cells exposed to either decorin or chondroitin sulfates migration and invasion decreased by more than 50%. Decorin led to a reversible, chondroitin-6-sulfate to an irreversible, cytosolic acidification of B16V cells. Interestingly, decorin lowered NHE1 activity whereas chondroitin-6-sulfate did not. Furthermore, decorin and chondroitin-6-sulfate also acidified the pH at the cell surface which might prevent migration due to strong adhesion. In conclusion, the present co-culture system is an appropriate tool to analyze migration, invasion, and MMP release depending on cell-matrix interactions and the crosstalk between the invasive cells and those surrounded by their self-made matrix. We show a so far unknown function of decorin and chondroitin-6-sulfate: their ability to inhibit B16V cell migration by intracellular acidification.
机译：体内,细胞是嵌入在一个环境由多个信息生成和维护和cell-matrix交互。真皮转移的黑素瘤细胞相互作用与细胞外基质(ECM)成纤维细胞。黑色素瘤(B16V)细胞和成纤维细胞迁移入侵,我们建立了培养系统成纤维细胞组成的,他们的collagen-rich矩阵和B16V细胞。细胞和成纤维细胞是由一个明确的表示发布和活动的增加matrix-metallo-protease-2。透露,在B16V细胞接触decorin或迁移和软骨素硫酸盐入侵下降了超过50%。一个可逆,chondroitin-6-sulfateB16V不可逆转,胞质酸化细胞。活动而chondroitin-6-sulfate没有。此外,decorin chondroitin-6-sulfate还酸化的pH值在细胞表面可能阻止移民由于附着力强。总之,目前的培养系统一个适当的工具来分析迁移,和MMP的入侵,根据发布cell-matrix交互和相声之间的浸润性细胞和那些包围他们白手起家的矩阵。decorin和未知函数chondroitin-6-sulfate:抑制的能力B16V由细胞内的细胞迁移酸化。

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