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首页> 外文期刊>Journal of Cellular Physiology >p8 expression controls pancreatic cancer cell migration, invasion, adhesion, and tumorigenesis.
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p8 expression controls pancreatic cancer cell migration, invasion, adhesion, and tumorigenesis.

机译：p8表达式控制胰腺癌细胞迁移、入侵、粘附、肿瘤发生。

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p8 is a stress gene whose activity is necessary for tumor development and progression. The acquisition of invasive properties by transformed cells is a key event in tumor development. In order to establish whether p8 is involved or not in this phenomenon, we assessed the capacity of p8 at influencing cell adhesion, migration, invasion, and tumorigenesis of pancreatic cancer cells. p8 expression was knocked down by a small interfering RNA (siRNA) in pancreatic cancer-derived Panc-1 and MiaPaCa-2 cells and subsequent changes in cell adhesion, migration, invasion, and tumorigenesis were assessed. Influence of p8 silencing on gene expression was analyzed using cDNA microarrays. The influence of inhibiting CDC42, one of the genes most over-expressed in p8-silenced cells, on the changes observed in p8-silenced cells was also evaluated. Finally, the tumorigenic capacities of Panc-1 cells transfected with control siRNA or p8 siRNA were compared by assessing their ability to form colonies in soft agar and to grow as xenografts in nude mice. Knocking-down p8 in pancreatic cancer cells in vitro decreased migration and invasion while increasing cell adhesion; over-expression produced the opposite effect. Knocking down CDC42 reversed almost completely the effects of silencing p8 in vitro. Finally, cells transfected with p8 siRNA were almost unable to form colonies in soft agar. In addition, p8-deficient Panc-1 cells did not develop tumors when injected subcutaneously in nude mice. In conclusion, p8 expression controls pancreatic cancer cell migration, invasion and adhesion, three processes required for metastasis, at least in part, through CDC42, a major regulator of cytoskeleton organization.
机译：p8压力基因的活动是必要的肿瘤发展和进步。收购入侵特性的改变细胞是肿瘤发展的关键事件。为了建立是否p8有关在这种现象中,我们评估的能力p8在影响细胞粘附、迁移、入侵,胰腺癌的肿瘤发生细胞。在胰腺核RNAcancer-derived Panc-1 MiaPaCa-2细胞后续更改在细胞粘附、迁移、入侵,肿瘤发生进行了评估。p8沉默对基因表达的影响使用互补脱氧核糖核酸微阵列分析。抑制CDC42,大部分的基因之一在p8-silenced细胞过度改变p8-silenced细胞也评估。与控制核或p8 Panc-1细胞转染核比较评估他们的能力形式殖民地在软琼脂和成长在裸鼠异种移植。胰腺癌细胞体外降低细胞迁移和入侵,同时增加附着力;的效果。完全沉默p8体外的影响。最后,细胞转染与p8核在软琼脂几乎无法形成殖民地。此外,p8-deficient Panc-1细胞没有开发肿瘤在皮下注射裸小鼠。胰腺癌细胞迁移,入侵和附着力,三个过程所需转移,至少在某种程度上,通过CDC42细胞骨架组织的主要监管机构。

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