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首页> 外文期刊>Journal of Cellular Physiology >Ascorbic acid-dependent GLUT3 inhibition is a critical step for switching neuronal metabolism.
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Ascorbic acid-dependent GLUT3 inhibition is a critical step for switching neuronal metabolism.

机译：抗坏血acid-dependent GLUT3抑制是一个开关神经元代谢的关键一步。

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Intracellular ascorbic acid is able to modulate neuronal glucose utilization between resting and activity periods. We have previously demonstrated that intracellular ascorbic acid inhibits deoxyglucose transport in primary cultures of cortical and hippocampal neurons and in HEK293 cells. The same effect was not seen in astrocytes. Since this observation was valid only for cells expressing glucose transporter 3 (GLUT3), we evaluated the importance of this transporter on the inhibitory effect of ascorbic acid on glucose transport. Intracellular ascorbic acid was able to inhibit (3)H-deoxyglucose transport only in astrocytes expressing GLUT3-EGFP. In C6 glioma cells and primary cultures of cortical neurons, which natively express GLUT3, the same inhibitory effect on (3)H-deoxyglucose transport and fluorescent hexose 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) was observed. Finally, knocking down the native expression of GLUT3 in primary cultured neurons and C6 cells using shRNA was sufficient to abolish the ascorbic acid-dependent inhibitory effect on uptake of glucose analogs. Uptake assays using real-time confocal microscopy demonstrated that ascorbic acid effect abrogation on 2-NBDG uptake in cultured neurons. Therefore, ascorbic acid would seem to function as a metabolic switch inhibiting glucose transport in neurons under glutamatergic synaptic activity through direct or indirect inhibition of GLUT3.
机译：细胞内抗坏血酸可以调节神经元葡萄糖利用率和休息之间活动时间。细胞内抗坏血酸抑制脱氧葡萄糖运输的主要文化皮质和海马神经元和HEK293细胞。星形胶质细胞。为细胞葡萄糖转运体表达3(GLUT3),我们评估的重要性转运体在抗坏血的抑制作用酸对葡萄糖运输。酸能够抑制(3)H-deoxyglucose只在星形胶质细胞表达运输GLUT3-EGFP。皮质神经元的文化,它本身表达GLUT3,相同的抑制作用(3) H-deoxyglucose运输和荧光己糖

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