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首页> 外文期刊>Journal of Cellular Physiology >Substance P stimulates the recovery of bone marrow after the irradiation.
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Substance P stimulates the recovery of bone marrow after the irradiation.

机译：P物质刺激骨髓的恢复辐照后。

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The therapeutic use of ionizing radiation (e.g., X-rays and gamma-rays) needs to inflict minimal damage on non-target tissue. Recent studies have shown that substance P (SP) mediates multiple activities in various cell types, including cell proliferation, anti-apoptotic responses, and inflammatory processes. The present study investigated the effects of SP on gamma-irradiated bone marrow stem cells (BMSCs). In mouse bone marrow extracts, SP prolonged activation of Erk1/2 and enhanced Bcl-2 expression, but attenuated the activation of apoptotic molecules (e.g., p38 and cleaved caspase-3) and down-regulated Bax. We also observed that SP-decreased apoptotic cell death and stimulated cell proliferation in gamma-irradiated mouse bone marrow tissues through TUNEL assay and PCNA analysis. To determine how SP affects bone marrow stem cell populations, mouse bone marrow cells were isolated and colony-forming unit (CFU) of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) was estimated. SP-pretreated ones showed higher CFUs of MSC and HSC than untreated ones. Furthermore, when SP was pretreated in cultured human MSC, it significantly decreased apoptotic cells at 48 and 72 h after gamma-irradiation. Compared with untreated cells, SP-treated human MSCs showed reduced cleavage of apoptotic molecules such as caspase-8, -9, -3, and poly ADP-ribose polymerase (PARP). Thus, our results suggest that SP alleviates gamma-radiation-induced damage to mouse BMSCs and human MSCs via regulation of the apoptotic pathway.
机译：治疗使用电离辐射(如x射线和伽马射线)需要造成最小破坏非目标组织。表明,P物质(SP)介导多种活动在不同的细胞类型,包括细胞增殖、抗凋亡反应和炎症过程。SP的影响调查gamma-irradiated骨髓干细胞(bmsc)。在小鼠骨髓提取,SP延长激活Erk1/2 bcl - 2和增强表达,但减毒的激活凋亡分子(例如,p38和裂解caspase-3)和衰减伯灵顿。观察到SP-decreased凋亡细胞死亡刺激细胞增殖gamma-irradiated小鼠骨髓组织通过TUNEL检测和PCNA的分析。确定SP如何影响骨髓干细胞人口,小鼠骨髓细胞分离和克隆形成单位(CFU)间充质干细胞(msc)和造血干细胞(hsc)估计。的显示cfu MSC和HSC高于未经处理的。在人工培养的MSC预处理在48和显著减少凋亡细胞辐照后72 h。未经处理的细胞,SP-treated人类msc减少了凋亡分子如的乳沟caspase-8 9 3,保利ADP-ribose聚合酶(PARP)。减轻gamma-radiation-induced损坏通过监管的鼠标bmsc与人类msc凋亡通路。

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