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首页> 外文期刊>Journal of Cellular Physiology >Embryonal carcinoma cell induction of miRNA and mRNA changes in co-cultured prostate stromal fibromuscular cells.
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Embryonal carcinoma cell induction of miRNA and mRNA changes in co-cultured prostate stromal fibromuscular cells.

机译：胚胎性癌细胞诱导microrna信使rna培养前列腺基质的变化纤维肌性的细胞。

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摘要

The prostate stromal mesenchyme controls organ-specific development. In cancer, the stromal compartment shows altered gene expression compared to non-cancer. The lineage relationship between cancer-associated stromal cells and normal tissue stromal cells is not known. Nor is the cause underlying the expression difference. Previously, the embryonal carcinoma (EC) cell line, NCCIT, was used by us to study the stromal induction property. In the current study, stromal cells from non-cancer (NP) and cancer (CP) were isolated from tissue specimens and co-cultured with NCCIT cells in a trans-well format to preclude heterotypic cell contact. After 3 days, the stromal cells were analyzed by gene arrays for microRNA (miRNA) and mRNA expression. In co-culture, NCCIT cells were found to alter the miRNA and mRNA expression of NP stromal cells to one like that of CP stromal cells. In contrast, NCCIT had no significant effect on the gene expression of CP stromal cells. We conclude that the gene expression changes in stromal cells can be induced by diffusible factors synthesized by EC cells, and suggest that cancer-associated stromal cells represent a more primitive or less differentiated stromal cell type.
机译：前列腺基质间质控制瀑特异性发展。基质间显示改变基因的表达而非癌症。与癌症相关的基质细胞正常组织基质细胞是未知的。背后的原因表达差异。在此之前,胚胎性癌细胞(EC)线,NCCIT,被我们研究基质感应财产。细胞非癌症(NP)和癌症(CP)从组织标本分离和培养NCCIT细胞trans-well格式排除异形的细胞接触。分析了基质细胞的基因阵列微rna (microRNA)和mRNA的表达。共培养,NCCIT细胞被发现改变microrna和NP基质细胞的信使rna表达一个像这样的CP基质细胞。对基因NCCIT没有显著影响CP基质细胞的表达。基质细胞的基因表达变化被诱导合成了可扩散的因素EC细胞,表明癌症相关基质细胞代表更原始的或更少不同基质细胞类型。

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