...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Serum-activated K and Cl currents underlay U87-MG glioblastoma cell migration.
【24h】

Serum-activated K and Cl currents underlay U87-MG glioblastoma cell migration.

机译：Serum-activated K和Cl电流衬底U87-MG胶质母细胞瘤细胞迁移。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glioblastoma cells in vivo are exposed to a variety of promigratory signals, including undefined serum components that infiltrate into high grade gliomas as result of blood-brain barrier breakdown. Glioblastoma cell migration has been further shown to depend heavily on ion channels activity. We have then investigated the modulatory effects of fetal calf serum (FCS) on ion channels, and their involvement in U87-MG cells migration. Using the perforated patch-clamp technique we have found that, in a subpopulation of cells (42%), FCS induced: (1) an oscillatory activity of TRAM-34 sensitive, intermediate-conductance calcium-activated K (IK(Ca) ) channels, mediated by calcium oscillations previously shown to be induced by FCS in this cell line; (2) a stable activation of a DIDS- and NPPB-sensitive Cl current displaying an outward rectifying instantaneous current-voltage relationship and a slow, voltage-dependent inactivation. By contrast, in another subpopulation of cells (32%) FCS induced a single, transient IK(Ca) current activation, always accompanied by a stable activation of the Cl current. The remaining cells did not respond to FCS. In order to understand whether the FCS-induced ion channel activities are instrumental to promoting cell migration, we tested the effects of TRAM-34 and DIDS on the FCS-induced U87-MG cell migration using transwell migration assays. We found that these inhibitors were able to markedly reduce U87-MG cell migration in the presence of FCS, and that their co-application resulted in an almost complete arrest of migration. It is concluded that the modulation of K and Cl ion fluxes is essential for the FCS-induced glioblastoma cell migration.
机译：胶质母细胞瘤细胞体内暴露于一个各种promigratory信号,包括未定义的血清渗透的组件高级别胶质瘤血脑的结果屏障崩溃。进一步证明很大程度上取决于离子渠道活动。胎牛血清(FCS)调节的影响离子通道,他们参与U87-MG细胞迁移。技术我们已经发现,在一个程序的细胞(42%)、FCS诱导:(1)一个振荡活动TRAM-34敏感,intermediate-conductance calcium-activated K(反向(Ca))频道,由钙之前振荡引起的FCS在这个细胞株;当前显示了——NPPB-sensitive Cl一个外向整流瞬时电流电压关系和缓慢,压敏电阻器失活。另一个族群的细胞(32%)FCS诱导一个单一的、瞬态动力学(Ca)当前的激活,总是伴随着一个稳定的激活Cl电流。FCS。FCS-induced离子通道的活动促进细胞迁移工具,我们测试TRAM-34和确实的影响使用transwell FCS-induced U87-MG细胞迁移迁移化验。能够显著减少U87-MG细胞迁移在FCS的存在,他们co-application导致几乎完成逮捕的移民。调制的K和Cl离子通量是至关重要的FCS-induced胶质母细胞瘤细胞的迁移。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号