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首页> 外文期刊>Journal of Cellular Physiology >5-Fluorouracil drug alters the microRNA expression profiles in MCF-7 breast cancer cells.
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5-Fluorouracil drug alters the microRNA expression profiles in MCF-7 breast cancer cells.

机译：5 -氟尿嘧啶药改变微表情概要MCF-7乳腺癌细胞。

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摘要

5-Fluorouracil (5-FU) is a classic chemotherapeutic drug that has been widely used for breast cancer treatment. Although aberrant expression of protein-coding genes was observed after 5-FU treatment, the regulatory mechanism remains poorly understood. MicroRNAs (miRNAs) are a newly identified class of small regulatory RNAs which play an important role in gene regulation at the post-transcriptional levels. Recent evidence suggests an important role of miRNAs in initiation, progression, and metastasis of human cancers. In this study, using a combined advanced microarray and quantitative real-time PCR (qRT-PCR) technology, we show that 5-FU significantly alters the global expression profile of miRNAs in vitro. After 48 h of treatment with a low dose (0.01 microM), 42 miRNAs were differentially expressed in MCF-7 breast cancer cell line. Of these, 23 miRNAs were up-regulated with up to 4.59-fold changes, while 19 were down-regulated with up to 1.89-fold changes. A majority of these miRNAs are associated with cancer development, progression, and metastasis. Target prediction and GO analysis suggest that these differentially expressed miRNAs potentially target many oncogenes, tumor suppressor genes and genes related to programmed cell death, activation of immune response, and cellular catabolic processes.
机译：5 -氟尿嘧啶(研究者用)是一个经典化疗药物已广泛应用乳腺癌的治疗。蛋白编码基因的表达被观察到研究者用治疗后,监管机制仍然知之甚少。一种新发现的小监管rna在基因调控中发挥着重要的作用吗在转录后水平。microrna的证据表明一个重要的角色人类发生、进展、转移的癌症。微阵列和定量实时PCR(存在)技术,我们表明,研究者用极大地改变了全球的表情microrna的体外。与低剂量(0.01 microM)治疗,42microrna在MCF-7差异表达乳腺癌细胞系。差异高达4.59倍的变化,19日下调了1.89倍的变化。与癌症有关开发、发展和转移。表明这些差异表达microrna潜在目标很多致癌基因,肿瘤抑制基因和基因相关的编程细胞死亡,激活免疫反应细胞分解代谢的过程。

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