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首页> 外文期刊>Journal of Cellular Physiology >Expression of estrogen receptor beta increases integrin alpha1 and integrin beta1 levels and enhances adhesion of breast cancer cells.
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Expression of estrogen receptor beta increases integrin alpha1 and integrin beta1 levels and enhances adhesion of breast cancer cells.

机译：表达雌激素受体β增加整合素α1和整合素beta1水平提高乳腺癌细胞的粘附。

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摘要

Estrogen effects on mammary gland development and differentiation are mediated by two receptors (ERalpha and ERbeta). Estrogen-bound ERalpha induces proliferation of mammary epithelial and cancer cells, while ERbeta is important for maintenance of the differentiated epithelium and inhibits proliferation in different cell systems. In addition, the normal breast contains higher ERbeta levels compared to the early stage breast cancers, suggesting that loss of ERbeta could be important in cancer development. Analysis of ERbeta-/- mice has consistently revealed reduced expression of cell adhesion proteins. As such, ERbeta is a candidate modulator of epithelial homeostasis and metastasis. Consequently, the aim of this study was to analyze estrogenic effects on adhesion of breast cancer cells expressing ERalpha and ERbeta. As ERbeta is widely found in breast cancer but not in cell lines, we used ERalpha positive T47-D and MCF-7 human breast cancer cells to generate cells with inducible ERbeta expression. Furthermore, the colon cancer cell lines SW480 and HT-29 were also used. Integrin alpha1 mRNA and protein levels increased following ERbeta expression. Integrin beta1-the unique partner for integrin alpha1-increased only at the protein level. ERbeta expression enhanced the formation of vinculin containing focal complexes and actin filaments, indicating a more adhesive potential. This was confirmed by adhesion assays where ERbeta increased adhesion to different extracellular matrix proteins, mostly laminin. In addition, ERbeta expression was associated to less cell migration. These results indicate that ERbeta affects integrin expression and clustering and consequently modulates adhesion and migration of breast cancer cells.
机译：雌激素对乳腺发育和影响分化是由两个受体介导的(ERalpha和ERbeta)。诱发乳腺上皮和扩散癌细胞,而ERbeta是重要的分化上皮和维护在不同的细胞抑制增殖系统。此外,正常乳腺包含更高ERbeta水平相比早期乳腺癌癌症,表明ERbeta可能的损失重要的癌症发展。ERbeta - / -小鼠一直显示减少细胞粘附蛋白的表达。ERbeta是上皮的候选人调制器体内平衡和转移。本研究旨在分析雌激素的影响乳腺癌细胞表达的粘附ERalpha ERbeta。乳腺癌而不是细胞系,我们使用ERalpha积极T47-D MCF-7人类乳房与诱导肿瘤细胞产生细胞ERbeta表达式。细胞系SW480和HT-29也使用。整合素α1信使rna和蛋白质含量增加后ERbeta表达式。独特的合作伙伴整合素alpha1-increased在蛋白质水平。vinculin含有焦的形成设施和肌动蛋白丝,表明更多胶粘剂的潜力。附着力化验ERbeta增加附着力不同的细胞外基质蛋白,层粘连蛋白。更少的细胞迁移有关。结果表明,ERbeta影响整合素表达和集群,因此调节粘附和迁移的乳腺癌细胞。

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