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首页> 外文期刊>Journal of Cellular Physiology >Endoglin is involved in BMP-2-induced osteogenic differentiation of periodontal ligament cells through a pathway independent of Smad-1/5/8 phosphorylation.
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Endoglin is involved in BMP-2-induced osteogenic differentiation of periodontal ligament cells through a pathway independent of Smad-1/5/8 phosphorylation.

机译：Endoglin BMP-2-induced成骨牙周韧带细胞的分化通过一个通路Smad-1/5/8独立的磷酸化。

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The periodontal ligament (PDL), a connective tissue located between the cementum of teeth and the alveolar bone of mandibula, plays a crucial role in the maintenance and regeneration of periodontal tissues. The PDL contains fibroblastic cells of a heterogeneous cell population, from which we have established several cell lines previously. To analyze characteristics unique for PDL at a molecular level, we performed cDNA microarray analysis of the PDL cells versus MC3T3-E1 osteoblastic cells. The analysis followed by validation by reverse transcription-polymerase chain reaction and immunochemical staining revealed that endoglin, which had been shown to associate with transforming growth factor (TGF)-beta and bone morphogenetic proteins (BMPs) as signaling modulators, was abundantly expressed in PDL cells but absent in osteoblastic cells. The knockdown of endoglin greatly suppressed the BMP-2-induced osteoblastic differentiation of PDL cells and subsequent mineralization. Interestingly, the endoglin knockdown did not alter the level of Smad-1/5/8 phosphorylation induced by BMP-2, while it suppressed the BMP-2-induced expression of Id1, a representative BMP-responsive gene. Therefore, it is conceivable that endoglin regulates the expression of BMP-2-responsive genes in PDL cells at some site downstream of Smad-1/5/8 phosphorylation. Alternatively, we found that Smad-2 as well as Smad-1/5/8 was phosphorylated by BMP-2 in the PDL cells, and that the BMP-2-induced Smad-2 phosphorylation was suppressed by the endoglin knockdown. These results, taken together, raise a possibility that PDL cells respond to BMP-2 via a unique signaling pathway dependent on endoglin, which is involved in the osteoblastic differentiation and mineralization of the cells.
机译：牙周韧带(PDL),一个连接组织位于牙齿和牙骨质下颌骨牙槽骨,扮演了一个至关重要的维护和再生的作用牙周组织。成纤维细胞的细胞的异质细胞人口,我们建立了前面几种细胞系。独特的PDL的分子特征层面上,我们互补脱氧核糖核酸微阵列分析执行PDL细胞与MC3T3-E1成骨细胞的细胞。分析验证的反向紧随其后聚合酶链反应和免疫化学染色显示,endoglin已被证明与转化生长因子β(TGF)和骨头形态形成蛋白(bmp)信号调节器在PDL细胞中大量表达但是没有在成骨细胞的细胞。BMP-2-induced endoglin极大地抑制PDL细胞和成骨细胞的分化随后的矿化。endoglin击倒没有改变的程度Smad-1/5/8 BMP-2引起的磷酸化,虽然它抑制BMP-2-induced表达式代表BMP-responsive Id1的基因。因此,可想而知,endoglin调节BMP-2-responsive的表达基因在PDL细胞在某些网站的下游Smad-1/5/8磷酸化。发现Smad-2 Smad-1/5/8一样磷酸化的BMP-2 PDL细胞,, BMP-2-induced Smad-2磷酸化抑制endoglin击倒。结果,综上所述,提高的可能性PDL细胞应对BMP-2通过一个独特的信号路径依赖endoglin,参与其中在成骨细胞的分化矿化的细胞。

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