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首页> 外文期刊>Journal of Cellular Physiology >Human testicular orphan receptor 4 enhances thyroid hormone receptor signaling.
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Human testicular orphan receptor 4 enhances thyroid hormone receptor signaling.

机译：人类睾丸孤儿受体4增强甲状腺激素受体信号。

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摘要

The thyroid hormone receptor (TR) and human testicular orphan receptor 4 (TR4) belong to the nuclear hormone receptor superfamily. They are ligand-dependent transcription factors. TR and TR4 bind to a similar thyroid response element (TRE), known as a direct repeat with four nucleotide spacing (DR4). This study examined the possible interaction or cross-talking between those two receptors. We hypothesized that protein-protein interaction between TR4 and TR may promote TR-mediated transcriptional activity. Glutathione S-transferase pull-down and immunoprecipitation assays showed direct interaction between TR and TR4. Electrophoretic mobility-shift assay demonstrated that TR and TR4 could co-occupy the same TRE. The interaction between TR4 and TR may enhance regulation of genes targeted by TR, such as furin, fibrinogen, cdk2 and p21 expression. We found that TR4 function is similar with TR as TR4 alone could regulate expression of some TR target genes, and could increase cell migration or inhibit cell proliferation. Importantly, the TR-dependent inhibition of cell proliferation and stimulation of cell migration are more enhanced in the HepG2-TR cells stably over-expressing TR4. Overall, TR4 not only has modulation abilities similar to TR but also can cross-talk with TR and promote the TR signaling pathway.
机译：甲状腺激素受体(TR)和人类睾丸孤儿受体4 (TR4)属于核激素受体超家族。ligand-dependent转录因子。TR4绑定到一个类似甲状腺响应元件(混乱关系),称为直接与四个重复核苷酸间距(DR4)。之间可能的相互作用或影响肺癌这两个受体。蛋白质之间的相互作用TR4和TR可以促进TR-mediated转录活动。谷胱甘肽S-transferase下拉,免疫沉淀反应化验显示直接TR和TR4之间的互动。mobility-shift试验表明,TR和TR4可能co-occupy相同的混乱关系。TR4之间和TR可能加强监管基因TR的目标,如furin、纤维蛋白原,cdk2和p21表达。函数与TR TR4相似就可以调节一些TR目标基因的表达可以增加细胞迁移或抑制细胞扩散。抑制细胞增殖和刺激细胞迁移更加强的HepG2-TR细胞过度表达TR4稳定。总的来说,TR4不仅调制能力类似于TR还可以用TR和相声促进TR信号通路。

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