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首页> 外文期刊>Journal of Cellular Physiology >Differential expression and cellular distribution of gamma-tubulin and betaIII-tubulin in medulloblastomas and human medulloblastoma cell lines.
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Differential expression and cellular distribution of gamma-tubulin and betaIII-tubulin in medulloblastomas and human medulloblastoma cell lines.

机译：微分表达式和细胞分布gamma-tubulin和betaIII-tubulin成神经管细胞瘤和人类成神经管细胞瘤细胞行。

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摘要

In previous studies, we have shown overexpression and ectopic subcellular distribution of gamma-tubulin and betaIII-tubulin in human glioblastomas and glioblastoma cell lines (Katsetos et al., 2006, J Neuropathol Exp Neurol 65:455-467; Katsetos et al., 2007, Neurochem Res 32:1387-1398). Here we determined the expression of gamma-tubulin in surgically excised medulloblastomas (n = 20) and in the human medulloblastoma cell lines D283 Med and DAOY. In clinical tissue samples, the immunohistochemical distribution of gamma-tubulin labeling was pervasive and inversely related to neuritogenesis. Overexpression of gamma-tubulin was widespread in poorly differentiated, proliferating tumor cells but was significantly diminished in quiescent differentiating tumor cells undergoing neuritogenesis, highlighted by betaIII-tubulin immunolabeling. By quantitative real-time PCR, gamma-tubulin transcripts for TUBG1, TUBG2, and TUBB3 genes were detected in both cell lines but expression was less prominent when compared with the human glioblastoma cell lines. Immunoblotting revealed comparable amounts of gamma-tubulin and betaIII-tubulin in different phases of cell cycle; however, a larger amount of gamma-tubulin was detected in D283 Med when compared with DAOY cells. Interphase D283 Med cells exhibited predominantly diffuse cytoplasmic gamma-tubulin localization, in addition to the expected centrosome-associated distribution. Robust betaIII-tubulin immunoreactivity was detected in mitotic spindles of DAOY cells. Our data indicate that overexpression of gamma-tubulin may be linked to phenotypic dedifferentiation (anaplasia) and tumor progression in medulloblastomas and may potentially serve as a promising tumor marker.
机译：在以前的研究中,我们显示超表达和异位的亚细胞分布gamma-tubulin betaIII-tubulin人类件和胶质母细胞瘤细胞系65:455-467;32:1387 - 1398)。gamma-tubulin手术切除成神经管细胞瘤(n = 20)和人类成神经管细胞瘤细胞系D283地中海和DAOY。临床组织样本,免疫组织化学gamma-tubulin分配标签普遍的和负相关neuritogenesis。在低分化广泛,肿瘤细胞增殖明显减少在静止区分肿瘤细胞接受neuritogenesis,凸显了betaIII-tubulin immunolabeling。实时PCR, gamma-tubulin成绩单TUBG1、TUBG2 TUBB3基因检测两个细胞系但是表达没有那么突出相比人类胶质母细胞瘤细胞行。gamma-tubulin和betaIII-tubulin不同细胞周期的阶段;gamma-tubulin在地中海D283当检测到相比之下,DAOY细胞。细胞表现出细胞质扩散为主gamma-tubulin本地化,除了预计centrosome-associated分布。健壮的betaIII-tubulin免疫反应性是检测到DAOY细胞的有丝分裂纺锤波。数据显示,超表达的gamma-tubulin可能与表型去分化(间)和肿瘤成神经管细胞瘤并可能发展可能作为一种很有前途的肿瘤标志物。

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