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首页> 外文期刊>Journal of Cellular Physiology >Both superficial and deep zone articular chondrocyte subpopulations exhibit the crabtree effect but have different basal oxygen consumption rates
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Both superficial and deep zone articular chondrocyte subpopulations exhibit the crabtree effect but have different basal oxygen consumption rates

机译：浅和深区关节表现出瑰柏翠的软骨细胞的细胞亚群但有不同的基底氧气的影响消费税率

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摘要

In the absence of in vivo measurements, the oxygen concentration within articular cartilage is calculated from the balance between cellular oxygen consumption and mass transfer. Current estimates of the oxygen tension within articular cartilage are based on oxygen consumption data from full-depth tissue samples. However, superficial and deep cell subpopulations of articular cartilage express intrinsic metabolic differences. We test the hypothesis that the subpopulations differ with respect to their intrinsic oxygen consumption rate. Chondrocytes from the full cartilage thickness demonstrate enhanced oxygen consumption when deprived of glucose, consistent with the Crabtree phenomena. Chondrocyte subpopulations differ in the prevailing availability of oxygen and glucose, which decrease with distance from the cartilage-synovial fluid interface. Thus, we tested the hypothesis that the oxygen consumption of each subpopulation is modulated by nutrient availability, by examining the expression of the Crabtree effect. The deep cells had a greater oxygen consumption than the superficial cells (Vmax of 6.6 compared to 3.2 fmol/cell/h), consistent with our observations of mitochondrial volume (mean values 52.0 vs. 36.4 μm3/cell). Both populations expressed the Crabtree phenomena, with oxygen consumption increasing ～2.5-fold in response to glycolytic inhibition by glucose deprivation or 2-deoxyglucose. Over 90% of this increase was oligomycin-sensitive and thus accounted for by oxidative phosphorylation. The data contributes towards our understanding of chondrocyte energy metabolism and provides information valuable for the accurate calculation of the oxygen concentration that the cells experience in vivo. The work has further application to the optimisation of bioreactor design and engineered tissues.
机译：在缺乏体内测量的情况下,氧气集中在关节软骨计算细胞之间的平衡耗氧量和传质。关节内的氧含量的估计软骨是基于氧气消耗数据从大吃刀组织样本。表面和深层细胞的亚种群关节软骨表达内在的代谢的差异。亚种群对他们的不同内在的耗氧率。从完整的软骨厚度展示增强的剥夺时的耗氧量葡萄糖,符合瑰柏翠的现象。软骨细胞亚种群的不同的可用性的氧气和葡萄糖,减少了距离cartilage-synovial流体界面。耗氧量的假说进行测试每个分组人口由营养调制可用性,通过检查的表达式瑰柏翠的效果。比表面的细胞耗氧量(6.6比3.2的Vmax fmol /电池/ h),符合我们的观察线粒体卷(平均值52.0 vs 36.4μm3 /单元)。人口表示瑰柏翠的现象,与耗氧量增加~ 2.5倍糖酵解反应抑制葡萄糖剥夺或2-deoxyglucose。oligomycin-sensitive因此增加占了氧化磷酸化。数据的贡献对我们的理解软骨细胞能量代谢和提供信息价值的准确计算氧浓度的细胞体内的经验。应用生物反应器的优化设计和策划组织。

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