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首页> 外文期刊>Journal of Cellular Physiology >Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor.
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Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor.

机译：葡萄糖胺促进成骨分化牙髓干细胞通过调制转化生长因子的类型我受体。

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摘要

Dental pulp stem cells (DPSCs) are clonogenic, self-renewing, and multi-potential DPSCs capable of differentiating into osteoblasts. In this study, primary cell cultures were obtained from human dental pulp tissue of developing third molars, and flow cytometry was used to sort the subpopulation of DPSCs with STRO-1 and CD146 double-positive expression (denoted "DPSCs"). It was noted that DPSCs exhibited superior clonogenic potential and osteogenic differentiation capability than the dental pulp cell subpopulation with STRO-1 and CD146 double-negative expression (denoted DPCs). Furthermore, a low concentration (0.005 mg/ml) of exogenous glucosamine (GlcN) was effective in promoting the early osteogenic differentiation of DPSCs through the transforming growth factor-beta receptor (TGF-betar) type I and Smads signal pathways, which upregulated the Runt-related transcription factor 2/core-binding factor alpha1 (Runx2/Cbfa1) and alkaline phosphatase at both the mRNA and protein levels. In the presence of osteogenic supplements, GlcN-treated DPSCs produced more mineralized-matrix deposition than did the untreated groups. Taken together, this study demonstrates the capacity of GlcN to promote the osteogenic differentiation of human DPSCs, and the underlying mechanism involves a TGF-betar-dependent Smad signal pathway.
机译：牙髓干细胞(DPSCs)是单独使用,自我更新,multi-potential DPSCs能干分化成成骨细胞。研究中,主要从细胞培养人类牙髓组织发展中第三臼齿,流式细胞仪是用来排序DPSCs STRO-1和CD146的族群double-positive表达式(表示“DPSCs”)。指出DPSCs表现出优越吗单独使用潜力和成骨的比牙髓的分化能力细胞亚群STRO-1和CD146双重否定表达(dpc)表示。此外,低浓度(0.005毫克/毫升)外源性葡萄糖胺(GlcN)是有效的促进的早期成骨分化DPSCs通过转化生长因子我和Smads信号受体(TGF-betar)类型通路,调节Runt-related转录因子α2 / core-binding因素(Runx2 / Cbfa1)和碱性磷酸酶信使rna和蛋白质的水平。成骨的补充剂,GlcN-treated DPSCsmineralized-matrix沉积多生产治疗组。研究表明GlcN的能力促进成骨分化的人类DPSCs,涉及到底层机制TGF-betar-dependent Smad信号通路。

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