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首页> 外文期刊>Journal of Cellular Physiology >Immunomodulatory properties of human periodontal ligament stem cells.
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Immunomodulatory properties of human periodontal ligament stem cells.

机译：人类牙周的免疫调节特性韧带干细胞。

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Tissue engineering utilizing periodontal ligament stem cells (PDLSCs) has recently been proposed for the development of new periodontal regenerative therapies. Although the use of autologous PDLSC transplantation eliminates the potential of a significant host immune response against the donor cells, it is often difficult to generate enough PDLSCs from one donor source due to the variation of stem cell potential between donors and disease state of each patient. In this study, we examined the immunomodulatory properties of PDLSCs as candidates for new allogeneic stem cell-based therapies. Human PDLSCs displayed cell surface marker characteristics and differentiation potential similar to bone marrow stromal stem cells (BMSSCs) and dental pulp stem cells (DPSCs). PDLSCs, BMSSCs, and DPSCs inhibited peripheral blood mononuclear cell (PBMNC) proliferation stimulated with mitogen or in an allogeneic mixed lymphocyte reaction (MLR). Interestingly, gingival fibroblasts (GFs) also suppressed allogeneic PBMNC proliferation under both assay conditions. PDLSCs, BMSSCs, DPSCs, and GFs exhibited non-cell contact dependent suppression of PBMNC proliferation in co-cultures using transwells. Furthermore, conditioned media (CM) derived from each cell type pretreated with IFN-gamma partially suppressed PBMNC proliferation when compared to CMs without IFN-gamma stimulation. In all of these mesenchymal cell types cultured with activated PBMNCs, the expression of TGF-beta1, hepatocyte growth factor (HGF) and indoleamine 2, 3-dioxygenase (IDO) was upregulated while IDO expression was upregulated following stimulation with IFN-gamma. These results suggest that PDLSCs, BMSSCs, DPSCs, and GFs possess immunosuppressive properties mediated, in part, by soluble factors, produced by activated PBMNCs. J. Cell. Physiol. 219: 667-676, 2009. (c) 2009 Wiley-Liss, Inc.
机译：利用牙周组织工程韧带干细胞(PDLSCs)最近提议发展的新的牙周再生疗法。自体移植PDLSC消除了重要的宿主免疫反应的潜力对供体细胞,通常很难产生足够的PDLSCs由于从一个供体来源干细胞之间潜在的变化捐助者和每个病人的疾病状态。研究中,我们检测了免疫调节的属性PDLSCs作为新的候选人同种异体干细胞疗法。PDLSCs显示细胞表面标记特点和分化潜能类似于骨髓基质干细胞(BMSSCs)和牙髓干细胞(DPSCs)。PDLSCs、BMSSCs DPSCs抑制外围血液单核细胞(PBMNC)扩散与有丝分裂原刺激或同种异体混合看不到淋巴细胞反应(MLR)。牙龈成纤维细胞(GFs)也抑制同种异体PBMNC扩散试验下条件。展出non-cell接触抑制的依赖PBMNC增殖培养使用transwells。来自每个细胞类型使用IFN-gamma部分抑制PBMNC扩散相比CMsIFN-gamma刺激。间充质细胞培养与激活PBMNCs TGF-beta1的表达,肝细胞生长因子(HGF)和吲哚胺2,3-dioxygenase (IDO)是调节而被罩表达调节刺激IFN-gamma。介导免疫抑制属性,在一定程度上,通过可溶性因子,由PBMNCs激活。j .细胞。Wiley-Liss公司。

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