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首页> 外文期刊>Journal of Cellular Physiology >Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics.
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Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics.

机译：在骨髓和脐带血的差异造血干细胞/祖细胞分裂动力学。

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Human hematopoietic stem/progenitor cells (HSC) isolated based upon specific patterns of CD34 and CD38 expression, despite phenotypically identical, were found to be functionally heterogeneous, raising the possibility that reversible expression of these antigens may occur during cellular activation and/or proliferation. In these studies, we combined PKH67 tracking with CD34/CD38 immunostaining to compare cell division kinetics between human bone marrow (BM) and cord blood (CB)-derived HSC expanded in a serum-free/stromal-based system for 14 days (d), and correlated CD34 and CD38 expression with the cell divisional history. CB cells began dividing 24 h earlier than BM cells, and significantly higher numbers underwent mitosis during the time in culture. By d10, over 55% of the CB-cells reached the ninth generation, whereas BM-cells were mostly distributed between the fifth and seventh generation. By d14, all CB cells had undergone multiple cell divisions, while 0.7-3.8% of BM CD34(+) cells remained quiescent. Furthermore, the percentage of BM cells expressing CD34 decreased from 60.8 +/- 6.3% to 30.6 +/- 6.7% prior to initiating division, suggesting that downmodulation of this antigen occurred before commencement of proliferation. Moreover, with BM, all primitive CD34(+)CD38(-) cells present at the end of culture arose from proliferating CD34(+)CD38(+) cells that downregulated CD38 expression, while in CB, a CD34(+)CD38(-) population was maintained throughout culture. These studies show that BM and CB cells differ significantly in cell division kinetics and expression of CD34 and CD38, and that the inherent modulation of these antigens during ex vivo expansion may lead to erroneous quantification of the stem cell content of the expanded graft.
机译：人类造血干/祖细胞(HSC)基于特定模式的CD34和孤立CD38的表情,尽管表型相同的,被发现的功能异构,提高的可能性可能发生可逆的这些抗原的表达在细胞活化和/或扩散。在这些研究中,我们结合PKH67跟踪CD34 / CD38疣状比较细胞分裂人类骨髓(BM)和线之间的动力学血液(CB)派生HSC在扩大血清/ stromal-based系统的14天(d),和CD34和CD38的表达相关细胞分割的历史。提前24小时BM细胞,明显更高的数字进行有丝分裂时在文化。达到第九代,而细胞群体大多分布在第五和第七代。经历了多个细胞分裂,而0.7 -3.8%的BM CD34(+)细胞保持静止。此外,细胞的百分比表达CD34下降从60.8 + / - 6.3%30.6 + / - 6.7%发起部门之前,这表明downmodulation抗原毕业典礼之前发生增殖。此外,随着BM,所有原始CD34 (+) CD38 (-)细胞在文化源自的结束增殖CD34 (+) CD38(+)细胞表达下调CD38表达式,而CBCD34 (+) CD38(-)人口维持在文化。和CB细胞在细胞方面有显著的差异部门动力学和CD34的表达CD38,这些固有的调制在体外扩张可能导致抗原错误的量化的干细胞的内容扩大的贪污。

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