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首页> 外文期刊>Journal of Cellular Physiology >Endogenous Jmjd6 gene product is expressed at the cell surface and regulates phagocytosis in immature monocyte-like activated THP-1 cells.
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Endogenous Jmjd6 gene product is expressed at the cell surface and regulates phagocytosis in immature monocyte-like activated THP-1 cells.

机译：内源性Jmjd6基因产物表达的细胞表面和调节吞噬作用不成熟的monocyte-like激活THP-1细胞。

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摘要

A jumonji domain containing gene 6 (Jmjd6), previously referred to as phosphatidylserine receptor (PSR) gene, plays an important role in cell differentiation and development of multiple organs, although mechanisms of its action are not known. The Jmjd6 gene product was initially identified as a membrane protein that participates in phagocytosis. However, the later findings that recombinant Jmjd6 in expression systems was targeted to the nucleus challenged the role of Jmjd6 as a membrane receptor. Using immunocytochemistry approach we studied the subcellular distribution of endogenous Jmjd6 protein in THP-1 cells activated with phorbol 12-myristate 13 acetate (PMA). We found that treatment with PMA stimulated Jmjd6 expression in the cytosol of activated cells. Furthermore, Jmjd6 initially appeared at the cell surface of immature phagocytes (1-2 days after activation) but then translocated into the nucleus of differentiated macrophage-like cells (5-9 days after activation). Anti-Jmjd6 antibodies suppressed the engulfment of dead cell corpses by THP-1 cells expressing the Jmjd6 at the cell surface. These data indicate that Jmjd6 serves as a membrane-associated receptor that regulates phagocytosis in immature macrophages but is dispensable for phagocytosis and has other functions when it is expressed in the cytosol and nucleus of mature macrophage-like cells.
机译：jumonji域包含基因6 (Jmjd6),以前称为磷脂酰丝氨酸受体(PSR)基因,起着重要的作用多种细胞分化和发展器官,但其作用机制已知的。确认为膜蛋白参与吞噬作用。发现重组Jmjd6表达式系统是针对细胞核挑战Jmjd6作为膜受体的作用。免疫细胞化学方法研究了内生Jmjd6亚细胞分布蛋白质与佛波醇THP-1细胞激活12-myristate 13乙酸酯(PMA)。治疗PMA刺激Jmjd6表达式激活细胞的胞质。Jmjd6最初出现在细胞表面不成熟的白细胞(激活后1 - 2天)但后来易位到的核心细胞分化macrophage-like(5 - 9天后激活)。抑制死细胞尸体的吞没的Jmjd6 THP-1细胞表达细胞表面。膜相关受体调节在不成熟的巨噬细胞吞噬作用,但可有可无的吞噬作用,还有其他的在胞质中表达和功能macrophage-like成熟细胞的细胞核。

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