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首页> 外文期刊>Journal of Cellular Physiology >Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
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Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.

机译：概要文件相关的液蛋白质释放分化和未分化的人类角化细胞。

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Our group has previously demonstrated the capacity of human keratinocytes to release 14-3-3sigma into conditioned medium through the mechanism of exosome externalization. In this study the release of other proteins through the same mechanism and the differences in the profiles of 14-3-3 proteins between differentiated (diff-K) and undifferentiated keratinocytes (undiff-K) were investigated. The stimulatory effect of other 14-3-3 isoforms on the expression of MMP-1 in dermal fibroblasts was also evaluated. Exosomes isolated from undiff-K (low Ca(2+)) and diff-K (high Ca(2+)) were subjected to proteomic and Western blot analysis. The results showed that more than 50 different cytoplasmic proteins including all seven 14-3-3 protein isoforms (beta, sigma, eta, epsilon, tau, zeta, and gamma) were released from diff-K through the mechanism of exosome externalization. However, in exosomes of undiff-K only four of the 14-3-3 protein isoforms (beta, eta, zeta, and gamma) were detected. Ca(2+) treatment increased the release of exosomes from undiff-K by at least two times relative to the control. Consistent with this finding, the stimulatory effect of exosomes containing 14-3-3sigma from diff-K had higher MMP-1 stimulatory effect in fibroblasts relative to those exosomes isolated from undiff-K. MMP-1 stimulatory effect of recombinant 14-3-3beta and eta, tested in this study, in dermal fibroblasts, suggests additional anti-fibrogenic factors other than 14-3-3sigma. In conclusion, keratinocytes release many proteins through the mechanism of exosome externalization from which some such as 14-3-3 isoforms may function as extracellular matrix (ECM) modulating factors for dermal fibroblasts. These findings revealed the presence of a novel mechanism by which keratinocytes can potentially interact with fibroblasts.
机译：我们组之前证明了能力人类的角化细胞释放14-3-3sigma成条件培养液的机制外来体外化。通过相同的释放其他蛋白质机制和概要文件的差异14-3-3蛋白之间的分化(diff-K)和未分化的角化细胞(undiff-K)被调查。其他14-3-3亚型金属蛋白酶- 1的表达在真皮成纤维细胞也被评估。液隔绝undiff-K(低Ca (2 +))diff-K(高钙(2 +))受到蛋白质组学和免疫印迹分析。,超过50种不同细胞质蛋白质包括所有七个14-3-3蛋白亚型(σβη,ε,τ,ζ,和γ)被释放从diff-K机制外来体的外化。14-3-3蛋白的undiff-K只有四个亚型(β,埃塔,ζ,γ)检测到。从undiff-K液至少两次相对于控制。发现,液的刺激效应包含从diff-K 14-3-3sigma更高金属蛋白酶- 1刺激成纤维细胞相对的影响液undiff-K隔绝。14-3-3beta和重组的刺激效应埃塔,测试在这项研究中,在真皮成纤维细胞,建议额外anti-fibrogenic其他因素14-3-3sigma。许多蛋白质释放的机制外来体等一些的外化14-3-3亚型可能作为细胞外矩阵(ECM)皮肤的调节因素成纤维细胞。一个新颖的机制的角质细胞可能与成纤维细胞。

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