...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Effects of morphine on testosterone levels in rat C6 glioma cells: modulation by anastrozole.
【24h】

Effects of morphine on testosterone levels in rat C6 glioma cells: modulation by anastrozole.

机译：吗啡对大鼠睾丸激素水平的影响阿那曲唑C6神经胶质瘤细胞:调制。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rat C6 glioma cells are commonly used to investigate the functions of glial cells. To evaluate the presence of testosterone and its metabolism in rat C6 glioma cells, we cultured them in media with or without the addition of testosterone propionate and anastrozole, a blocker of aromatase, the enzyme needed to transform testosterone into estradiol. The same procedure was repeated with morphine (10 and 100 microM), known to decrease testosterone levels in the brain (in rats) and plasma (in rats and humans). Confluent cells were exposed to the test media for 48 h and then collected. Cell pellets were used to determine testosterone by radioimmunoassay. The C6 cells contained detectable levels of testosterone and the levels increased with the addition of testosterone to the medium. Aromatase blockage by anastrozole increased cellular levels of testosterone regardless of the addition of exogenous testosterone. Both concentrations of morphine dose-dependently decreased testosterone levels in the C6 cells; this effect was also present with the contemporary administration of anastrozole. Our findings show that testosterone is present in rat C6 glioma cells and can be metabolized by aromatase. Moreover, the presence of morphine in the culture medium strongly decreased testosterone, demonstrating that the glia would be a target of the morphine-induced hypogonadal effect.
机译：一般用于大鼠C6胶质瘤细胞研究神经胶质细胞的功能。睾酮的存在及其评估鼠C6神经胶质瘤细胞的新陈代谢,我们讲究的在媒体有或没有的丙酸睾酮和阿那曲唑拦截器的芳香化酶,这种酶需要睾酮转化为雌二醇。过程与吗啡(10和100年重复microM),睾丸激素水平降低大脑(老鼠)和等离子体(在老鼠和人类)。媒体48 h,然后收集。被用来确定睾酮放射免疫检定法。可检测水平的睾酮水平增加的睾酮的媒介。增加细胞的睾丸激素水平无论添加外源睾丸激素。剂量依赖性降低睾丸激素水平C6细胞;阿那曲唑的现代政府。我们的研究结果表明,睾酮存在于大鼠C6胶质瘤细胞,可以代谢芳香化酶。培养基强烈降低了睾酮,表明神经胶质吗啡hypogonadal的目标的效果。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号