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首页> 外文期刊>Journal of Cellular Physiology >Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
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Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.

机译：Immunophenotypic人类关节的变化软骨细胞单层培养中反映真诚的去分化而不是放大的祖细胞。

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摘要

In this study, a time-course comparison of human articular chondrocytes (HAC) and bone marrow-derived mesenchymal stem cells (MSC) immunophenotype was performed in order to determine similarities/differences between both cell types during monolayer culture, and to identify HAC surface markers indicative of dedifferentiation. Our results show that dedifferentiated HAC can be distinguished from MSC by combining CD14, CD90, and CD105 expression, with dedifferentiated HAC being CD14+/CD90bright/CD105dim and MSC being CD14-/CD90dim/CD105bright. Surface markers on MSC showed little variation during the culture, whereas HAC showed upregulation of CD90, CD166, CD49c, CD44, CD10, CD26, CD49e, CD151, CD51/61, and CD81, and downregulation of CD49a, CD54, and CD14. Thus, dedifferentiated HAC appear as a bona fide cell population rather than a small population of MSC amplified during monolayer culture. While most of the HAC surface markers showed major changes at the beginning of the culture period (Passage 1-2), CD26 was upregulated and CD49a downregulated at later stages of the culture (Passage 3-4). To correlate changes in HAC surface markers with changes in extracellular matrix gene expression during monolayer culture, CD14 and CD90 mRNA levels were combined into a new differentiation index and compared with the established differentiation indices based on the ratios of mRNA levels of collagen type II to I (COL2/COL1) and of aggrecan to versican (AGG/VER). A correlation of CD14/CD90 ratio at the mRNA and protein level with the AGG/VER ratio during HAC dedifferentiation in monolayer culture validated CD14/CD90 as a new membrane and mRNA based HAC differentiation index.
机译：在这项研究中,人类的时间进程的比较关节软骨细胞(HAC)和骨头骨髓来源间充质干细胞(MSC)为了执行immunophenotype确定这两个产品的异同之处在单层培养细胞类型,表面标记识别工厂的说明去分化。HAC肉瘤可以区分开来MSC通过结合CD14, CD90、CD105表达式,HAC肉瘤CD14 + / CD90bright / CD105dim和MSCCD14 - / CD90dim / CD105bright。在文化,显示小变化而HAC显示upregulation CD90、CD166CD49c, CD44, CD10 CD26、CD49e CD151, CD51/61,CD49a,差别的研究,对这些CD54,CD14。的细胞群,而不是一个小的人口在单层MSC放大文化。初的重大变化文化时期(通道1 - 2),CD26在后来调节和CD49a表达下调阶段的文化(通过3 - 4)。改变肝表面标记的变化细胞外基质中基因表达单层培养、CD14和CD90 mRNA水平组合成一个新的分化指数和相比之下,建立了分化基于mRNA水平的比率指标胶原蛋白II型的我(COL2 / COL1)和aggrecanversican (gg /版本)。信使rna和蛋白质含量的比例去分化在gg /版本比在工厂单层培养验证CD14 / CD90作为一个新的膜和mRNA HAC分化索引。

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