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首页> 外文期刊>Journal of Cellular Physiology >Docosahexaenoic acid is more potent inhibitor of osteoclast differentiation in RAW 264.7 cells than eicosapentaenoic acid.
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Docosahexaenoic acid is more potent inhibitor of osteoclast differentiation in RAW 264.7 cells than eicosapentaenoic acid.

机译：二十二碳六烯酸的有效抑制剂生264.7中破骨细胞分化的细胞二十碳五烯酸。

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摘要

Fish oil rich in n-3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) protects inflammation induced bone loss in chronic inflammatory diseases like rheumatoid arthritis, periodontitis, and osteoporosis. EPA and DHA differentially regulate functional parameters and gene expression in different cell types. One of the risk factors for bone loss in inflammatory bone diseases is the elevation of bone-resorbing osteoclasts and a very few studies so far have indicated that attenuation of osteoclastogenesis might be one of the mechanisms by which n-3 PUFA exert its effect on bone loss protection. However, the precise mechanism underlying this process remains unclear. Receptor activator of NF-kappaB ligand (RANKL) is known to be the most critical mediator of osteoclastogenesis. Therefore, in this study, we examined the differential effect of EPA and DHA on RANKL-stimulated osteoclastogenesis and RANKL signaling using a murine monocytic cell line RAW 264.7. DHA was found to inhibit osteoclast differentiation, activation and function more potently than EPA. The differential potential also closely correlated with the inhibition of osteoclast-specific genes like tartrate resistant acid phosphatase, cathepsin K, calcitonin receptor, matrix metalloproteinase-9 expression and osteoclast-specific transcription factor, c-Fos, as well as osteotropic proinflammatory cytokine, TNF-alpha to a greater extent with DHA than EPA. Further, pretreatment of RAW 264.7 cells with DHA also showed significantly reduced activation of NF-kappaB and p38MAPK than EPA. Our findings suggest that DHA may be much more effective than EPA in alleviating RANKL induced proinflammatory cytokine production, intracellular signaling activation, thereby decreasing osteoclast activation and bone resorption.
机译：鱼油富含n - 3多不饱和脂肪酸,尤其是二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)保护炎症诱导慢性炎症的骨质流失类风湿性关节炎等疾病,牙周炎,骨质疏松症。不同的调节功能参数和基因表达在不同的细胞类型。炎症的骨质疏松的危险因素骨疾病是微的高程破骨细胞和极少数研究迄今为止表明osteoclastogenesis衰减可能是n - 3 PUFA的机制之一发挥其对骨质疏松的影响的保护。然而,精确的机制过程仍不清楚。NF-kappaB配体(RANKL)是已知的最osteoclastogenesis的关键中介。因此,在这项研究中,我们调查了EPA和DHA的微分效应RANKL-stimulated osteoclastogenesis和RANKL使用小鼠单核细胞的细胞系的原始信号264.7. 分化、激活和功能更多比美国环保署强有力地。也与抑制密切相关osteoclast-specific基因抗酒石酸酸性磷酸酶,组织蛋白酶K,降钙素受体,矩阵metalloproteinase-9表达式osteoclast-specific转录因子,c-Fos以及osteotropic促炎细胞因子,tnf与DHA在更大程度上美国环境保护署。细胞与DHA也表现出显著降低激活NF-kappaB和p38MAPK环保局。研究结果表明,DHA可能更多比EPA在有效减轻RANKL诱导促炎细胞因子的生产、胞内信号激活,从而减少破骨细胞活化和骨头吸收。

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