• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >An anti-apoptotic protein, Hax-1, inhibits the HIV-1 rev function by altering its sub-cellular localization.
【24h】

An anti-apoptotic protein, Hax-1, inhibits the HIV-1 rev function by altering its sub-cellular localization.

机译：抗凋亡蛋白,Hax-1,抑制hiv - 1转速函数通过改变其亚细胞本地化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The human immunodeficiency virus type 1 (HIV-1) Rev protein facilitates the nuclear export of viral mRNA containing the Rev response element (RRE). Although several host proteins co-operating with Rev in viral RNA export have been reported, little is known about the innate host defense factors that Rev overcomes to mediate the nuclear export of unspliced viral mRNAs. We report here that an anti-apoptotic protein, HS1-associated protein X-1 (Hax-1), a target of HIV-1 Vpr, interacts with Rev and inhibits its activity in RRE-mediated gene expression. Co-expression of Sam68 emancipates Rev activity from Hax-1-mediated inhibition. Hax-1 does not bind to RRE RNA by itself, but inhibits Rev from binding to RRE RNA in vitro. The impact of Hax-1 on Rev/RRE interactions in vitro correlates well with the reduced level of RRE-containing mRNA in vivo. Immunofluorescence studies further reveal that Hax-1 and Rev are cytoplasmic and nuclear proteins, respectively, when expressed independently. However, in Hax-1 co-expressing cells, Rev is translocated from the nucleus to the cytoplasm, where it is co-localized with Hax-1 in the cytoplasm. We propose that over-expression of Hax-1, possibly through binding to Rev, may interfere with the stability/export of RRE-containing mRNA and target the RNA for degradation.
机译：人类免疫缺陷病毒1型(hiv - 1)牧师蛋白促进核出口信使rna病毒包含转速响应元素(RRE)。配合牧师在病毒RNA的出口据报道,先天是知之甚少宿主防御因素,克服了牧师调解unspliced病毒的核出口信使rna。蛋白质,HS1-associated蛋白质x - 1 (Hax-1)hiv - 1的目标冲程体积,与牧师和交互在RRE-mediated基因抑制其活性表达式。牧师从Hax-1-mediated抑制活动。Hax-1不绑定到RRE RNA,但是从绑定RRE RNA体外抑制转速。Hax-1在牧师/ RRE交互的影响体外相关的水平RRE-containing mRNA体内。研究进一步表明,Hax-1和牧师细胞质和核蛋白质,分别当独立表达。共同表达细胞,加速转移的细胞质细胞核,它在哪里硝唑与Hax-1在细胞质中。建议Hax-1表达,可能通过绑定到牧师,可能会干扰/出口RRE-containing mRNA和稳定目标的RNA降解。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号