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首页> 外文期刊>Journal of Cellular Physiology >Integrin Cytoplasmic domain-Associated Protein-1 (ICAP-1) promotes migration of myoblasts and affects focal adhesions.
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Integrin Cytoplasmic domain-Associated Protein-1 (ICAP-1) promotes migration of myoblasts and affects focal adhesions.

机译：整合素胞质domain-Associated蛋白1(ICAP-1)促进成肌细胞的迁移和影响焦粘连。

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摘要

Integrin Cytoplasmic domain-Associated Protein-1 (ICAP-1) binds specifically to the beta1 integrin subunit cytoplasmic domain. We observed that RNAi-induced knockdown of ICAP-1 reduced migration of C2C12 myoblasts on the beta1 integrin ligand laminin and that overexpression of ICAP-1 increased this migration. In contrast, migration on the beta3 integrin ligand vitronectin was not affected. ICAP-1 knockdown also greatly diminished migration of microvascular endothelial cells on collagen. The number of central focal adhesions in C2C12 cells on laminin was reduced by ICAP-1 knockdown and increased by ICAP-1 overexpression. Previously, we demonstrated that ICAP-1 binds to the ROCK-I kinase and translocates ROCK-I to the plasma membrane. We show here that the ROCK kinase inhibitor Y27362 reduces migration on laminin and causes a loss of central focal adhesions, similarly as ICAP-1 knockdown. ICAP-1 and ROCK were co-immune-precipitated from C2C12 cells, and in cells that overexpressed ICAP-1, YFP-ROCK was translocated to membrane ruffles. These results indicate that ICAP-1 regulates beta1 integrin-dependent cell migration by affecting the pattern of focal adhesion formation. This is likely due to ICAP-1-induced translocation of ROCK to beta1 integrin attachment sites.
机译：整合素胞质domain-Associated蛋白1(ICAP-1)结合具体的beta1整合素亚基胞质域。RNAi-induced击倒的ICAP-1减少beta1迁移C2C12成肌细胞整合素配体层粘连蛋白和超表达ICAP-1增加这个迁移。迁移在beta3整合素配体vitronectin没有影响。也大大减少了迁移的微血管内皮细胞在胶原蛋白。数量的中心焦点在C2C12细胞粘连对层粘连蛋白减少ICAP-1击倒增加了ICAP-1超表达。我们证明ICAP-1结合巨石激酶和把巨石等离子体膜。移民对层粘连蛋白抑制剂Y27362减少中央焦点粘连造成损失,同样作为ICAP-1击倒。从C2C12细胞co-immune-precipitated,在细胞过表达ICAP-1 YFP-ROCK转移到膜褶边。表明ICAP-1调节beta1integrin-dependent细胞迁移的影响粘着斑形成的模式。可能由于ICAP-1-induced易位岩石beta1整合素连接的网站。

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