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首页> 外文期刊>Journal of Cellular Physiology >Soluble receptor inhibits leptin transport.
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Soluble receptor inhibits leptin transport.

机译：可溶性瘦素受体抑制交通。

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Evidence both from mice and cultured cells suggests an important role of soluble leptin receptors in obesity and leptin signaling. However, the direct effects of soluble receptors on leptin uptake by cells are not clear. This study shows that soluble leptin receptors antagonize the permeation of leptin across the mouse blood-brain barrier by reducing the binding and endocytosis of leptin. This is illustrated by analysis of radioactively labeled and fluorescent-tagged leptin in normal mice and in cultured cells overexpressing various forms of leptin receptors. Three constructs of soluble leptin receptors were generated in this study: ObRe (805 aa), ObR839, and ObR852. (125)I-leptin was injected intravenously and its influx rate from blood to brain determined by multiple-time regression analysis. Pre-incubation with ObR839 caused a significant reduction of leptin influx across the blood-brain barrier. Endocytosis assays and fluorescent image analysis further showed that ObRe, ObR839, and ObR852 failed tomediate leptin internalization and trafficking within the cells. Instead, these soluble receptors inhibited surface binding and endocytosis of leptin. Thus, we provide novel direct evidence both in vivo and in vitro that soluble receptors of leptin serve as antagonists of the transport of leptin.
机译：证据来自老鼠和培养细胞表明可溶性瘦素的一个重要的角色肥胖和瘦素受体的信号。然而,可溶性受体的直接影响在瘦素吸收细胞尚不清楚。研究表明,可溶性瘦素受体对抗渗透的瘦素减少小鼠血脑屏障的绑定瘦素和内吞作用。放射性标记和分析瘦素在正常小鼠和荧光标记培养细胞overexpressing各种形式的瘦素受体。瘦素受体在这项研究中生成:是静脉注射和流入率从血液到大脑由灾害回归分析。显著降低瘦素引起的大量涌入穿过血脑屏障。进一步分析和荧光图像分析表明ObRe、ObR839 ObR852失败了tomediate瘦素内化和贩卖在细胞内。绑定和受体抑制表面内吞作用的瘦素。体内和体外的直接证据可溶性瘦素受体作为拮抗剂瘦素的运输。

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