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首页> 外文期刊>Journal of Cellular Physiology >Immortalized mouse articular cartilage cell lines retain chondrocyte phenotype and respond to both anabolic factor BMP-2 and pro-inflammatory factor IL-1.
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Immortalized mouse articular cartilage cell lines retain chondrocyte phenotype and respond to both anabolic factor BMP-2 and pro-inflammatory factor IL-1.

机译：不灭的鼠标关节软骨细胞系保持软骨细胞表型和应对合成代谢因素BMP-2和促炎因子

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摘要

Articular cartilage chondrocytes help in the maintenance of tissue homeostasis and function of the articular joint. Study of primary chondrocytes in culture provides information closely related to in vivo functions of these cells. Limitations in the primary culture of chondrocytes have lead to the development of cells lines that serve as good surrogate models for the study of chondrocyte biology. In this study, we report the establishment and characterization of chondrocyte cell lines, MM-Sv/HP and MM-Sv/HP-2 from mouse articular cartilage. Cells were isolated from mouse femoral head articular cartilage, immortalized and maintained in culture through numerous passages. The morphology of the cells was from fibroblastic to polygonal in nature. Gene expression studies using quantitative PCR (Q-PCR) were performed on cells in monolayer culture and cells embedded in a three-dimensional alginate matrix. Stimulation of cells in monolayer culture with anabolic factor, BMP-2, resulted in increased gene expression of the extracellular matrix molecules, aggrecan and type II collagen and their regulator transcription factor, Sox9. Treatment by pro-inflammatory IL-1 resulted in increased gene expression of catabolic effectors including Aggrecanases (ADAMTS4, ADAMTS5), MMP-13 and nitric oxide synthase (Nos2). Cells in alginate treated with BMP-2 resulted in increased synthesis of proteoglycan which was released into the conditioned media on IL-1 stimulation. Western analysis of conditioned media showed the presence of Aggrecanase-cleaved aggrecan fragments. In summary, MM-Sv/HP and MM-Sv/HP-2 show preservation of important characteristics of articular chondrocytes as examined under multiple culture conditions and would provide a useful reagent in the study of chondrocyte biology.
机译：关节软骨软骨细胞的帮助维护组织的体内平衡和功能关节的关节。软骨细胞在文化提供了信息体内的功能密切相关细胞。软骨细胞导致的发展细胞,作为良好的代理模型软骨细胞生物学研究。建立和研究中,我们报告对软骨细胞的细胞系,MM-Sv /惠普和MM-Sv HP-2从鼠标关节软骨。头关节软骨,不灭的维护文化通过许多段落。从成纤维细胞的细胞的形态多边形的性质。使用定量PCR (Q-PCR)进行在单层培养细胞,细胞嵌入一个三维的海藻酸矩阵。在单层培养细胞的合成代谢因素,BMP-2,导致增加的基因的细胞外基质分子的表达,aggrecan和II型胶原蛋白及其监管机构转录因子、Sox9。促炎il - 1导致增加的基因表达式的分解效果器,包括Aggrecanases (ADAMTS4, ADAMTS5), MMP-13 and一氧化氮合酶(Nos2)。对待BMP-2导致增加蛋白多糖的合成释放到条件媒体对il - 1刺激。西方的媒体分析显示存在Aggrecanase-cleaved aggrecan碎片。显示保存的重要特征检查下多个关节软骨细胞培养条件,并将提供一个有用的试剂在软骨细胞生物学的研究。

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