...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Wheat germ agglutinin stains dispersed post-golgi vesicles after treatment with the cytokinesis inhibitor psychosine.
【24h】

Wheat germ agglutinin stains dispersed post-golgi vesicles after treatment with the cytokinesis inhibitor psychosine.

机译：麦胚凝集素染色post-golgi驱散治疗后囊泡胞质分裂抑制剂吐根素。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The galactosylsphingosine psychosine (Psy) is one of the sphingolipids and induce the formation of multinuclear cells in several cell lines by inhibiting cytokinesis. In the present report, we show that intracellular organelles, including wheat germ agglutinin (WGA)-positive vesicles and early endosomes, are selectively dispersed by Psy. WGA is a conventional Golgi marker and WGA-positive vesicles appeared to co-localize with the Golgi apparatus in untreated cells. Psy treatment induced the dispersal of WGA-positive vesicles without affecting the structure of the Golgi apparatus, resulting in discrimination of WGA-positive vesicles from the Golgi apparatus. In sharp contrast to this effect of Psy, WGA-positive vesicles were not affected by brefeldin A treatment, which induced the disappearance of the Golgi apparatus. Immunostaining with anti-TGN46 antibodies revealed that a large portion of the WGA-positive vesicles were derived from the trans-Golgi network. Notably, the dispersed WGA-positive vesiclesdid not stain with anti-syntaxin 6, another marker of the trans-Golgi network. During cytokinesis, WGA-positive vesicles in the cytoplasm decreased, and WGA staining accumulated at the cleavage furrow, which was apparently inhibited by the presence of Psy. These data suggest that the transport of WGA-positive vesicles to the cleavage furrow is associated with the progression of cytokinesis.
机译：的galactosylsphingosine吐根素(小组)就是其中之一鞘脂类和诱导的形成多核细胞在一些细胞系抑制胞质分裂。表明细胞内细胞器,包括麦胚凝集素(WGA)阳性的囊泡早期核内体,选择性分散了小组。硝唑WGA-positive囊泡出现未经处理的细胞中高尔基体。治疗诱导WGA-positive的传播囊泡而不影响的结构高尔基体,导致歧视的WGA-positive从高尔基体小泡。在本小组的影响形成鲜明对比,WGA-positive囊泡没有影响brefeldin治疗,诱导高尔基体的消失。疣状与anti-TGN46抗体透露,WGA-positive的很大一部分囊泡是源自于trans-Golgi网络。vesiclesdid不染色anti-syntaxin 6,trans-Golgi网络的另一个标志。胞质分裂,WGA-positive囊泡胞浆减少,WGA的染色积累在卵裂沟,这很明显抑制小组的存在。表明WGA-positive的运输囊泡的卵裂沟相关联与胞质分裂的进展。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号