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首页> 外文期刊>Journal of Cellular Physiology >Osteoclast stimulatory transmembrane protein (OC-STAMP), a novel protein induced by RANKL that promotes osteoclast differentiation.
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Osteoclast stimulatory transmembrane protein (OC-STAMP), a novel protein induced by RANKL that promotes osteoclast differentiation.

机译：破骨细胞刺激跨膜蛋白(OC-STAMP)小说RANKL引起的蛋白质促进破骨细胞分化。

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Microarray and real-time RT-PCR were used to examine expression changes in primary bone marrow cells and RAW 264.7 cells in response to RANKL. In silico sequence analysis was performed on a novel gene which we designate OC-STAMP. Specific siRNA and antibodies were used to inhibit OC-STAMP RNA and protein, respectively, and tartrate-resistant acid phosphatase (TRAP)+ multinucleated osteoclasts were counted. Antibodies were used to probe bone tissues and western blots of RAW cell extracts +/- RANKL. cDNA overexpression constructs were transfected into RAW cells and the effect on RANKL-induced differentiation was studied. OC-STAMP was very strongly up-regulated during osteoclast differentiation. Northern blots and sequence analysis revealed two transcripts of 2 and 3.7 kb differing only in 3'UTR length, consistent with predictions from genome sequence. The mRNA encodes a 498 amino acid, multipass transmembrane protein that is highly conserved in mammals. It has little overall homology to other proteins. The carboxy-terminal 193 amino acids, however, are significantly similar to the DC-STAMP family consensus sequence. DC-STAMP is a transmembrane protein required for osteoclast precursor fusion. Knockdown of OC-STAMP mRNA by siRNA and protein inhibition by antibodies significantly suppressed the formation of TRAP+, multinucleated cells in differentiating osteoclast cultures, with many TRAP+ mononuclear cells present. Conversely, overexpression of OC-STAMP increased osteoclastic differentiation of RAW 264.7 cells. We conclude that OC-STAMP is a previously unknown, RANKL-induced, multipass transmembrane protein that promotes the formation of multinucleated osteoclasts.
机译：微阵列和实时rt - pcr被用来原发性骨髓检查表达变化264.7细胞和原始细胞RANKL的回应。在硅片进行序列分析我们指定OC-STAMP小说基因。核和抗体被用来抑制OC-STAMP RNA和蛋白质,分别tartrate-resistant酸性磷酸酶(陷阱)+多核破骨细胞数。骨骼组织和抗体被用来探测西方的原始细胞提取物+ / - RANKL洇。互补脱氧核糖核酸超表达结构被转染RANKL-induced原始细胞和效果分化进行了研究。在破骨细胞表达强烈上调分化。分析显示两个成绩单2和3.7 kb只在3 'utr长度不同,一致从基因组序列的预测。编码498个氨基酸,多通道的跨膜在哺乳动物中高度保守的蛋白质。几乎没有其它整体同源蛋白质。然而,carboxy-terminal 193个氨基酸明显类似于DC-STAMP家庭吗共识序列。破骨细胞前体融合所需的蛋白质。可拆卸的小干扰rna和蛋白质OC-STAMP信使rna的抗体抑制可显著抑制+陷阱的形成,多核细胞区分破骨细胞文化,许多陷阱+单核细胞。过度的OC-STAMP增加监测264.7分化的原始细胞。OC-STAMP是未知的,RANKL-induced,多通道的跨膜蛋白促进多核的形成破骨细胞。

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