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首页> 外文期刊>Journal of Cellular Physiology >Ultrasound stimulates MMP-13 expression through p38 and JNK pathway in osteoblasts.
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Ultrasound stimulates MMP-13 expression through p38 and JNK pathway in osteoblasts.

机译：通过超声波刺激MMP-13表达式p38和物在成骨细胞通路。

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摘要

It has been shown that ultrasound (US) stimulation accelerates fracture healing, bone maturation, and remodeling in the animal models and in clinical studies. One of the major factor involves in remodeling process is matrix metalloproteinases (MMPs) such as MMP-13 that has been shown to degrade the native interstitial collagens in several tissues. Here we found that US stimulation increased the secretion of MMP-13 in cultured rat osteoblasts, as shown by zymographic analysis. US stimulation also increased the mRNA level of MMP-13, c-Fos, and c-Jun. Cycloheximide (an inhibitor of protein translocation) and actinomycin D (an inhibitor of gene transcription) did not inhibit the MMP-13, c-Fos, and c-Jun mRNA expression, suggesting that such expression does not require de novo protein synthesis and not change their stabilities. p38 inhibitor, SB203580 or JNK inhibitor, SP600125 but not ERK inhibitor, PD98059 attenuated the US-induced MMP-13, c-Fos, and c-Jun expression; these results were further substantiated by transfecting with the dominant negative mutants of p38 or JNK. The binding of c-Fos and c-Jun to the AP-1 element on the MMP-13 promoter and the enhancement of AP-1 luciferase activity was enhanced by US stimulation. Taken together, our results provide evidence that US stimulation increases MMP-13 expression through p38 and JNK signaling pathway to regulate bone remodeling.
机译：它已经表明,超声(美国)刺激加速骨折愈合,骨成熟,并在动物模型和重构临床研究。包括在装修过程中矩阵金属蛋白酶(mmp) MMP-13等降低本地间隙胶原蛋白在一些组织。我们刺激MMP-13的分泌增加在培养的大鼠成骨细胞,如图所示zymographic分析。增加的mRNA水平MMP-13 c-Fos,c-Jun。易位)和放线菌素D(抑制剂基因转录)没有抑制MMP-13,c-Fos, c-Jun mRNA表达,这表明这样表达不需要从头蛋白质合成,而不是改变他们的稳定性。抑制剂,SB203580或物抑制剂,SP600125但不是ERK抑制剂,PD98059减毒US-induced MMP-13、c-Fos c-Jun表达式;这些结果进一步证实使转染与占主导地位的消极的突变体p38或物。AP-1元素MMP-13启动子和AP-1荧光素酶活性的增强增强了我们的刺激。结果提供的证据表明,我们的刺激增加MMP-13表达式通过p38和物信号通路调节骨重塑。

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