Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.

Reed SA; Johnson SE

首页> 外文期刊>Journal of Cellular Physiology >Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.

【24h】

Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.

机译：马脐带血包含一个人口表达Oct4和分化的干细胞为中胚层和内胚层的细胞类型。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Mesenchymal stem cells (MSCs) offer promise as therapeutic aids in the repair of tendon, ligament, and bone damage suffered by sport horses. The objective of the study was to identify and characterize stem-like cells from newborn foal umbilical cord blood (UCB). UCB was collected and MSC isolated using human reagents. The cells exhibit a fibroblast-like morphology and express the stem cell markers Oct4, SSEA-1, Tra1-60 and Tra1-81. Culture of the cells in tissue-specific differentiation media leads to the formation of cell types characteristic of mesodermal and endodermal origins. Chondrogenic differentiation reveals proteoglycan and glycosaminoglycan synthesis as measured histochemically and Sox9 and collagen 2A1 gene transcription. Osteocytes capable of mineral deposition, osteonectin and Runx2 transcription were evident. Hepatogenic cells formed from UCBs express albumin and cytokeratin 18. Multinucleated myofibers that express desmin were observed indicating partial differentiation into mature muscle cells. Interestingly, conventional human protocols for UCB differentiation into adipocytes were unsuccessful in foal UCB and adult horse adipose-derived MSC. These results demonstrate that equine UCB can be induced to form multiple cell types that underlie their value for regenerative medicine in injured horses. In addition, this work suggests that subtle differences exist between equine and human UCB stem cells.

机译：间充质干细胞(msc)提供承诺治疗艾滋病在肌腱的修复,韧带和骨损伤遭受运动马。识别和描述干细胞样细胞小马驹新生儿脐血(UCB)。收集和MSC孤立使用人类的试剂。表现出纤维母细胞形态和表达干细胞标记Oct4、SSEA-1Tra1-60和tra1 - 81。组织分化媒体导致的细胞类型特征的形成中胚层和内胚层的起源。分化显示蛋白多糖和粘多糖的合成来衡量组织化学地和Sox9和胶原蛋白2 a1基因转录。沉积,骨粘连蛋白和Runx2转录是很明显的。表达蛋白和细胞角蛋白18。多核肌纤维肌间线蛋白表达观察表明偏微分法成熟的肌肉细胞。UCB分化成人类协议脂肪细胞在马驹UCB和失败成年马脂肪MSC。证明马UCB可以诱导多种细胞类型,造成他们的形式价值再生医学在受伤马。马和人类之间存在细微的差异UCB干细胞。

著录项

来源
《Journal of Cellular Physiology》 |2008年第2期|329-336|共8页
作者
Reed SA; Johnson SE;
展开▼
作者单位

Department of Animal Sciences, University of Florida, Gainesville, Florida 32611, USA.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Stem Cells; Adipocytes; cell typePOU5F1 geneCell DifferentiationBiological MarkersCord bloodNewborn InfantEquidaeEquusglycosaminoglycan metabolismEndoderm Cell;

机译：干细胞,脂肪细胞;细胞typePOU5F1 geneCellDifferentiationBiological MarkersCordmetabolismEndoderm细胞;

相似文献

外文文献
中文文献

1. Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem cells derived from human umbilical cord blood cells in vitro [J] . Lei Chen, Zhongguo Zhang, Bing Chen, 中国神经再生研究：英文版 . 2011,第013期
2. Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem cells derived from human umbilical cord blood cells in vitro [J] . Lei Chen, Guozhen Hui, Zhongguo Zhang, 中国神经再生研究（英文版） . 2011,第013期
3. Factors inducing human umbilical cord blood-derived mesenchymal stem cells to differentiate into neuron-like cells [J] . Nawei Zhang, Fengqing Ji 中国神经再生研究（英文版） . 2006,第006期
4. Editor＇s Choice —Umbilical cord blood mesenchymal stem cell differentiation and transplantation in neural regeneration [J] . 无中国神经再生研究：英文版 . 2011,第022期
5. https://researchopenworld.com/development-of-synthetic-cell-differentiation-agent-formulations-for-the-prevention-and-therapy-of-cancer-via-targeting-of-cancer-stem-cells/# [O] . 2019

机译：https://researchopenworld.com/development-of-synthetic-cell-differentiation-agent-formulations-for-the-prevention-and-therapy-of-cancer-via-targeting-of-cancer-stem-cells/#

Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.

摘要

著录项

相似文献

相关主题

期刊订阅