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首页> 外文期刊>Journal of Cellular Physiology >Cancer testis antigens in human melanoma stem cells: expression, distribution, and methylation status.
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Cancer testis antigens in human melanoma stem cells: expression, distribution, and methylation status.

机译：人类黑色素瘤茎癌睾丸抗原细胞:表达、分布和甲基化的地位。

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摘要

Neoplastic populations with stem cell potential have been most recently identified in human cutaneous melanoma, and initially characterized for their phenotypic profile. Being melanoma stem cells (MSC) the most desirable target of therapeutic intervention, we asked whether they express the epigenetically-regulated cancer testis antigens (CTA) on which melanoma immunotherapy is increasingly focusing. Reverse transcription-PCR analyses identified the presence of the large majority of investigated CTA (i.e., MAGE, GAGE, NY-ESO, and SSX families) in different MSC populations. MSC expressed MAGE-A proteins as detected by western blot; noteworthy, the distribution of MAGE-A proteins was highly homogeneous within given MSC populations as shown by confocal immunofluorescence. Promoter methylation studies unveiled a homogeneously-demethylated MAGE-A3 promoter that paired MAGE-A3 expression in MSC. Altogether these findings demonstrate that MSC can be efficiently targeted by CTA-directed immunotherapeutic approaches, and suggest that epigenetic patterns most likely drive the expression of CTA in MSC as previously shown for melanoma cells.
机译：肿瘤人群与干细胞的潜力最近发现在人类呢皮肤的黑色素瘤和最初的特点为他们的表型。细胞(MSC)最理想的目标治疗性干预,我们问他们是否表达epigenetically-regulated癌症黑色素瘤的睾丸抗原(CTA)免疫治疗越来越关注。一分析确定了存在大部分的调查CTA(例如,法师,计、NY-ESO和SSX家庭)在不同的MSC的人口。MAGE-A蛋白质免疫印迹检测;值得注意的,MAGE-A蛋白的分布在给定的MSC高度均匀人口由共焦如图所示免疫荧光。公布了一项homogeneously-demethylated MAGE-A3启动子成对MAGE-A3表达式在MSC。这些发现表明,MSC可以有效的目标CTA-directed吗免疫治疗的方法和建议表观遗传模式最有可能的驱动CTA在MSC正如前面所示的表情黑色素瘤细胞。

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