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首页> 外文期刊>Journal of Cellular Physiology >Decorin enhances the proliferation and differentiation of myogenic cells through suppressing myostatin activity.
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Decorin enhances the proliferation and differentiation of myogenic cells through suppressing myostatin activity.

机译：Decorin扩散和增强肌原性的细胞的分化抑制肌肉生长抑制素的活动。

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Decorin, a small leucine-rich proteoglycan, plays an important role in the regulation of cell growth. Our recent study has shown that immobilized decorin in the collagen matrix sequesters myostatin into the extracellular matrix and prevents its inhibitory action to myoblast proliferation in vitro. However, it still remains unclear whether free decorin could affect the proliferation and differentiation of myogenic cells by regulating myostatin activity. In the present study, we generated stable clonal C2C12 myoblasts that were over-expressing decorin, and showed that decorin over-expressing cells had an increased rate of proliferation as compared to control cells. Decorin over-expressing cells formed multi-giant hypertrophic myotubes with an elongated morphology and larger size as compared to control cells, although the initiation of differentiation in decorin over-expressing cells was somewhat delayed as compared to control cells. Western blot analysis demonstrated that MyoD expression in decorin over-expressing cells was lower than that in control cells until 12 h after induction to differentiate. At 48-h differentiation, the expressions of MyoD, p21 and myogenin were dramatically increased in cells that over-expressed decorin. Furthermore, we revealed that over-expression of decorin suppressed the activity of myostatin endogenously synthesized in C2C12 myoblasts and attenuated the signaling of exogenous myostatin. Consistent with these results, knock-down of decorin impairs C2C12 myoblast growth by increasing the sensitivity to exogenous myostatin. These results clearly show that decorin enhances the proliferation and differentiation of C2C12 myoblasts through suppressing myostatin activity.
机译：Decorin,小富亮氨酸蛋白多糖、戏剧一个重要的角色在细胞的调节增长。固定化decorin胶原基质扣押肌肉生长抑制素到细胞外矩阵和防止其抑制作用成肌细胞体外增殖。仍然不清楚decorin可以自由增殖和分化的影响肌原性的细胞通过调节肌肉生长抑制素的活动。在目前的研究中,我们生成稳定的克隆C2C12成肌细胞过度表达decorin,表明decorin过度表达细胞增殖率增加而控制细胞。过度表达细胞形成multi-giant肥厚性肌管拉长形态和更大的规模比控制细胞,虽然开始分化在decorin有点过度表达细胞控制细胞延迟相比。污点分析表明MyoD表达式在低于decorin过度表达细胞控制细胞诱导后直到12 h来区分。p21的表达MyoD,和myogenin在细胞显著增加过度decorin。表达decorin镇压了肌肉生长抑制素活性内生的合成C2C12成肌细胞和衰减的信号外生肌肉生长抑制素。结果,decorin损害C2C12混战成肌细胞增长通过增加灵敏度外生肌肉生长抑制素。decorin增强扩散和分化的C2C12成肌细胞抑制肌肉生长抑制素的活动。

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