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首页> 外文期刊>Journal of Cellular Physiology >The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells.
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The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells.

机译：的tetraspanin CD9调节表皮生长在癌症细胞因子受体信号。

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摘要

CD9 is a member of the tetraspanins, and has been shown to be involved in a variety of cellular activities such as migration, proliferation, and adhesion. In addition, it has been known that CD9 can associate with other proteins. Here we demonstrated the physical and functional association of CD9 with epidermal growth factor receptor (EGFR) on MKN-28 cells. Double-immunofluorescent staining and immunoprecipitation demonstrated the complex formation of CD9-EGFR and CD9-beta(1) integrin, and that both complexes are colocalized on the cell surface especially at the cell-cell contact site. Anti-CD9 monoclonal antibody ALB6 induced a dotted or patch-like aggregation pattern of both CD9-EGFR and CD9-beta(1) integrin. The internalization of EGFR after EGF-stimulation was significantly enhanced by the treatment with ALB6. CD9 can associate with EGFR in hepatocellular carcinoma cells (HepG2/CD9) and Chinese hamster ovary cancer cells (CHO-HER/CD9), which were transfected with pTJ/human EGFR/CD9. Furthermore expression of CD9 specifically attenuated EGFR signaling in CHO-HER/CD9 cells through the down regulation of surface expression of EGFR. These results suggest that CD9 might have an important role that attenuates EGFR signaling. Therefore, CD9 not only associates EGFR but also a new regulator, which may affect EGF-induced signaling in cancer cells.
机译：CD9是tetraspanins成员,参与多种细胞活动,比如迁移、扩散和附着力。可以将与其他蛋白质。证明了物理和功能CD9协会与表皮生长因子MKN-28细胞受体(EGFR)。Double-immunofluorescent染色和免疫沉淀反应了复杂形成CD9-EGFR和CD9-beta(1)整合素,和这两种复合物与在细胞表面特别是信息联系网站。虚线或patch-like聚合模式两种CD9-EGFR和CD9-beta(1)整合素。表皮生长因子受体在EGF-stimulation内化极大地增强了治疗ALB6。肝癌细胞HepG2 / CD9和中国仓鼠卵巢肿瘤细胞(CHO-HER / CD9),这是人类表皮生长因子受体/ CD9转染pTJ /。此外CD9的表达减毒在CHO-HER / CD9细胞表皮生长因子受体信号通过表面表达的调节表皮生长因子受体。有一个重要的角色,变弱表皮生长因子受体信号。表皮生长因子受体也是一个新的监管机构,这可能影响EGF-induced信号在癌症细胞。

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