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首页> 外文期刊>Journal of Cellular Physiology >Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development.
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Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development.

机译：线粒体转录因子的表达在猪配子形成和(TFAM)胚胎发育。

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摘要

Mitochondrial transcription factor A (TFAM) is responsible for stability, maintenance, and transcriptional control of mitochondrial DNA (mtDNA). We have studied the expression and distribution of TFAM in the gametes and preimplantation embryos of the domestic pig (Sus scrofa). We hypothesized that TFAM is not present in the boar sperm mitochondria to reduce the possibility of paternal mtDNA propagation in the progeny. In contrast, we anticipated that Tfam gene is expressed in a developmental stage-dependent manner in porcine oocytes and embryos. The appropriate TFAM band of 25 kDa was detected by Western blotting in ejaculated boar spermatozoa, as well as in porcine oocytes and zygotes. Boar sperm extracts also displayed several bands >25 kDa suggestive of post-translational modification by ubiquitination, confirmed by affinity purification of ubiquitinated proteins. TFAM immunoreactivity was relegated to the sperm tail principal piece and sperm head in fully differentiated spermatozoa. The content of Tfam mRNA increased considerably from the germinal vesicle to blastocyst stage and also between in vitro fertilized and cultured blastocysts compared to in vivo-derived blastocysts. TFAM protein accumulated in the oocytes during maturation and was reduced by proteolysis after fertilization. This pattern was not mirrored in parthenogenetically activated oocytes and zygotes reconstructed by SCNT, suggesting deviant processing of TFAM protein and transcript after oocyte/embryo manipulation. Thus, TFAM may exert a critical role in porcine gametogenesis and preimplantation embryo development. Altogether, our data on the role of TFAM in mitochondrial function and inheritance have broad implications for cell physiology and evolutionary biology.
机译：线粒体转录因子(TFAM)负责稳定、维护和线粒体DNA的转录控制(mtDNA)。TFAM配子和的分布胚胎植入前的国内猪(Sus的胚胎scrofa)。在公猪精子线粒体减少父母mtDNA传播的可能性后代。基因表达的发育猪卵母细胞和stage-dependent方式胚胎。被西方墨点法在射精野猪猪卵母细胞和精子,以及受精卵。几个乐队> 25 kDa暗示的翻译修饰的泛素化,证实了亲和力净化ubiquitinated蛋白质。免疫反应性是精子尾部主段和精子的头分化精子。从胚mRNA大幅增加泡到胚泡阶段之间也体外受精和培养的胚泡而在vivo-derived胚泡。蛋白质积累在卵母细胞成熟后,降低了蛋白质水解受精。卵母细胞孤雌激活和受精卵由SCNT重建,显示异常TFAM蛋白和转录后的处理卵母细胞或胚胎操作。在猪配子形成与一个关键的角色胚胎发育。我们的数据在线粒体TFAM所扮演的角色函数和继承有广泛的影响细胞生理和进化生物学。

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